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Literature summary for 2.2.1.6 extracted from

  • Chang, A.K.; Duggleby, R.G.
    Expression, purification and characterization of Arabidopsis thaliana acetohydroxyacid synthase (1997), Biochem. J., 327, 161-169.
No PubMed abstract available
Search for more literature for 2.2.1.6

Cloned(Commentary)

Cloned (Comment) Organism
cloned into the bacterial expression vector pT7-7 and expressed in Escherichia coli BL21(DE3) Arabidopsis thaliana

Inhibitors

Inhibitors Comment Organism Structure
chlorsulphuron
-
 Arabidopsis thaliana
Ile insensitive to Arabidopsis thaliana
imazapyr
-
 Arabidopsis thaliana
Leu insensitive to Arabidopsis thaliana
Val no inhibition Arabidopsis thaliana

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
additional information
-
 additional information non-hyperbolic substrate-saturation curve, involving interaction between the active sites of the dimer Arabidopsis thaliana
8.01
-
 pyruvate Km-value of the first active site Arabidopsis thaliana
100
-
 pyruvate Km-value of the second active site Arabidopsis thaliana

Metals/Ions

Metals/Ions Comment Organism Structure
Mg2+ required Arabidopsis thaliana

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
61000
-
 2 * 61000, SDS-PAGE Arabidopsis thaliana
63864
-
 2 * 63864, ion spray MS analysis Arabidopsis thaliana
109000
-
 gel filtration in absence of FAD Arabidopsis thaliana
113000
-
 gel filtration in presence of FAD Arabidopsis thaliana

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
pyruvate Arabidopsis thaliana first enzyme unique to biosynthesis of the branched chain amino acids Val, Leu, and Ile ?
-
 ?

Organism

Organism UniProt Comment Textmining
Arabidopsis thaliana
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
 Arabidopsis thaliana

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
7.88
-
-
 Arabidopsis thaliana

Storage Stability

Storage Stability Organism
-70°C, 4% loss of activity after 3 weeks with three freeze-thaw cycles, 8% loss of activity after 5 weeks with five freeze-thaw cycles, 9-10% loss of activity after 6 months with no intervening freeze-thaw cycle Arabidopsis thaliana
4°C, 10% loss of activity after 3 weeks, 23% loss of activity after 5 weeks Arabidopsis thaliana

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
pyruvate
-
 Arabidopsis thaliana 2-acetolactate + CO2
-
 ?
pyruvate first enzyme unique to biosynthesis of the branched chain amino acids Val, Leu, and Ile Arabidopsis thaliana ?
-
 ?

Subunits

Subunits Comment Organism
dimer 2 * 61000, SDS-PAGE Arabidopsis thaliana
dimer 2 * 63864, ion spray MS analysis Arabidopsis thaliana

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
6.5 8.5
-
 Arabidopsis thaliana

Cofactor

Cofactor Comment Organism Structure
FAD required as cofactor Arabidopsis thaliana
thiamine diphosphate required as cofactor Arabidopsis thaliana