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Literature summary for 2.2.1.6 extracted from
- Acetohydroxy acid synthetase with a pH optimum of 7.5 from Neurospora crassa mitochondria: characterization and partial purification (1972), J. Bacteriol., 112, 453-464.
General Stability
| General Stability | Organism |
|---|---|
| stabilized by pyruvate, 2-mercaptoethanol or dithiothreitol | Neurospora crassa |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| 2-oxobutanoate | - |
Neurospora crassa |  |
| PCMB | - |
Neurospora crassa | |
| Val | noncompetitive; pH-dependent inhibition | Neurospora crassa | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 3.2 | - |
pyruvate | - |
Neurospora crassa | |
| 17 | - |
pyruvate | - |
Neurospora crassa | |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| mitochondrion | - |
Neurospora crassa | 5739 | - |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Mg2+ | Mn2+ or Mg2+ required | Neurospora crassa | |
| Mn2+ | Mn2+ or Mg2+ required | Neurospora crassa | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 456000 | - |
predominant form, an enzyme form of 110000 Da also detected, gel filtration | Neurospora crassa |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Neurospora crassa | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Neurospora crassa |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 0.4075 | - |
- |
Neurospora crassa |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| pyruvate | - |
Neurospora crassa | 2-acetolactate + CO2 | - |
? | |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
- |
- |
freezing and subfreezing temperatures are severely damaging | Neurospora crassa |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7.5 | - |
- |
Neurospora crassa |
Cofactor
| Cofactor | Comment | Organism | Structure |
|---|---|---|---|
| thiamine diphosphate | required as cofactor | Neurospora crassa | |
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Release 2023.1 (January 2023)
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