Cloned (Comment) | Organism |
---|---|
the gene is cloned the gene with an attached hexahistidine (His6) tag at the C terminus | Serratia marcescens |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
pyruvate + (E)-oct-2-enal | Serratia marcescens | first step in the biosynthesis of the red pigment, prodigiosin | (S)-3-acetyloctanal + CO2 | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Serratia marcescens | Q5W251 | - |
- |
Purification (Comment) | Organism |
---|---|
- |
Serratia marcescens |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
2-oxobutanoate + (3E)-4-(4-chlorophenyl)but-3-en-2-one | - |
Serratia marcescens | ? | - |
? | |
additional information | PigD catalyzes 1,2-additions using 2-oxoacids as donor substrates and aldehydes as acceptors. In the case of ketones as acceptors PigD catalyzes the 1,4-addition. Although pyruvate is the preferred donor substrate, 2-oxobutanoate is also employed using (E)-4-(4-chlorophenyl)-but-3-en-2-one as an acceptor | Serratia marcescens | ? | - |
? | |
pyruvate + (2E)-1,3-diphenylprop-2-en-1-one | the PigD-catalyzed reaction yields 3% 1,4-carboligation product, with an enantiomeric excess above 99% | Serratia marcescens | (3R)-1,3-diphenylpentane-1,4-dione + CO2 | - |
? | |
pyruvate + (2E)-3-(2-hydroxyphenyl)-1-phenylprop-2-en-1-one | the PigD-catalyzed reaction yields 39% 1,4-carboligation product, with an enantiomeric excess above 99% | Serratia marcescens | (3R)-3-(2-hydroxyphenyl)-1-phenylpentane-1,4-dione + CO2 | - |
? | |
pyruvate + (2E)-3-(4-methoxyphenyl)-1-phenylprop-2-en-1-one | - |
Serratia marcescens | (3R)-3-(4-methoxyphenyl)-1-phenylpentane-1,4-dione + CO2 | - |
? | |
pyruvate + (3E)-4-(4-chlorophenyl)but-3-en-2-one | the PigD-catalyzed reaction yields 13% 1,4-carboligation product, with an enantiomeric excess above 99% | Serratia marcescens | (3R)-3-(4-chlorophenyl)hexane-2,5-dione + CO2 | - |
? | |
pyruvate + (3E)-4-(4-hydroxy-3-methoxyphenyl)but-3-en-2-one | - |
Serratia marcescens | (3R)-3-(4-hydroxy-3-methoxyphenyl)hexane-2,5-dione + CO2 | - |
? | |
pyruvate + (3E)-4-(furan-2-yl)but-3-en-2-one | 10% conversion | Serratia marcescens | (3R)-3-(furan-2-yl)hexane-2,5-dione + CO2 | - |
? | |
pyruvate + (3E)-4-(thiophen-2-yl)but-3-en-2-one | 15% conversion | Serratia marcescens | (3S)-3-(thiophen-2-yl)hexane-2,5-dione + CO2 | - |
? | |
pyruvate + (3E)-4-phenylbut-3-en-2-one | the PigD-catalyzed reaction yields 7% 1,4-carboligation product, with an enantiomeric excess above 95% | Serratia marcescens | (3R)-3-phenylhexane-2,5-dione + CO2 | - |
? | |
pyruvate + (3E)-dec-3-en-2-one | the PigD-catalyzed reaction yields 30% 1,4-carboligation product, with an enantiomeric excess above 99% | Serratia marcescens | (3S)-3-hexylhexane-2,5-dione + CO2 | - |
? | |
pyruvate + (3E)-non-3-en-2-one | the PigD-catalyzed reaction yields 38% 1,4-carboligation product, with an enantiomeric excess above 99% | Serratia marcescens | (3S)-3-pentylhexane-2,5-dione + CO2 | - |
? | |
pyruvate + (E)-oct-2-enal | - |
Serratia marcescens | (S)-3-acetyloctanal + CO2 | - |
? | |
pyruvate + (E)-oct-2-enal | first step in the biosynthesis of the red pigment, prodigiosin | Serratia marcescens | (S)-3-acetyloctanal + CO2 | - |
? | |
pyruvate + 3-phenylbut-3-en-2-one | - |
Serratia marcescens | (3R)-3-phenylhexane-2,5-dione + CO2 | - |
? | |
pyruvate + S-[2-(acetylamino)ethyl] (2E)-oct-2-enethioate | highest conversion of all tested substrates into the 1,4-addition product | Serratia marcescens | S-[2-(acetylamino)ethyl] (2E)-oct-2-enethioate + CO2 | - |
? |
Synonyms | Comment | Organism |
---|---|---|
pigD | - |
Serratia marcescens |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
30 | - |
assay at | Serratia marcescens |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
thiamine diphosphate | required | Serratia marcescens |