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Literature summary for 2.1.3.6 extracted from

  • Llacer, J.L.; Polo, L.M.; Tavarez, S.; Alarcon, B.; Hilario, R.; Rubio, V.
    The gene cluster for agmatine catabolism of Enterococcus faecalis: study of recombinant putrescine transcarbamylase and agmatine deiminase and a snapshot of agmatine deiminase catalyzing its reaction (2007), J. Bacteriol., 189, 1254-1265.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 2.1.3.6

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli Enterococcus faecalis
gene ef0732, gene cluster organization, DNA and amino acid sequence determination and analysis, overexpression of His-tagged enzyme in Escherichia coli Enterococcus faecalis

Crystallization (Commentary)

Crystallization (Comment) Organism
purified recombinant enzyme in presence of inhibitor N-(phosphonoacetyl)-putrescine, X-ray diffraction structure determination and analysis at 3.0 A resolution Enterococcus faecalis
using the sparse-matrix sampling vapor-diffusion method. The addition of N-(phosphonoacetyl)-putrescine to the crystallization drop strongly improves the results of the crystallization Enterococcus faecalis

Inhibitors

Inhibitors Comment Organism Structure
N-(phosphonoacetyl)-putrescine i.e. PAPU, highly potent and selective inhibitor Enterococcus faecalis
phosphonoacetyl-L-aspartate
-
 Enterococcus faecalis
phosphonoacetyl-L-ornithine
-
 Enterococcus faecalis

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.0023
-
 putrescine
-
 Enterococcus faecalis
0.058
-
 Carbamoyl phosphate
-
 Enterococcus faecalis

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
40000
-
 gel filtration Enterococcus faecalis

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
carbamoyl phosphate + L-ornithine Enterococcus faecalis low activity, reaction of EC 2.1.3.3 phosphate + N-carbamoyl-L-ornithine
-
 ?
carbamoyl phosphate + L-ornithine Enterococcus faecalis ATCC 700802 low activity, reaction of EC 2.1.3.3 phosphate + N-carbamoyl-L-ornithine
-
 ?
carbamoyl phosphate + putrescine Enterococcus faecalis
-
 phosphate + N-carbamoyl-putrescine
-
 ?
carbamoyl phosphate + putrescine Enterococcus faecalis ATCC 700802
-
 phosphate + N-carbamoyl-putrescine
-
 ?
additional information Enterococcus faecalis the enzyme catalyzes the second of three steps of ATP synthesis from agmatine ?
-
 ?
additional information Enterococcus faecalis ATCC 700802 the enzyme catalyzes the second of three steps of ATP synthesis from agmatine ?
-
 ?

Organism

Organism UniProt Comment Textmining
Enterococcus faecalis
-
-
-
Enterococcus faecalis
-
 strain SD10, gene ef0732
-
Enterococcus faecalis ATCC 700802
-
 strain SD10, gene ef0732
-

Purification (Commentary)

Purification (Comment) Organism
recombinant His-tagged enzyme from Escherichia coli by nickel affinity chromatography Enterococcus faecalis

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
460
-
 non-recombinant enzyme from Enterococcus faecalis Enterococcus faecalis
597
-
 recombinant enzyme from Enterococcus faecalis Enterococcus faecalis

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
carbamoyl phosphate + cadaverine only weak activity with cadaverine a substrate Enterococcus faecalis phosphate + N-carbamoylcadaverine
-
 ?
carbamoyl phosphate + cadaverine only weak activity with cadaverine a substrate Enterococcus faecalis ATCC 700802 phosphate + N-carbamoylcadaverine
-
 ?
carbamoyl phosphate + L-ornithine low activity, reaction of EC 2.1.3.3 Enterococcus faecalis phosphate + N-carbamoyl-L-ornithine
-
 ?
carbamoyl phosphate + L-ornithine low activity, reaction of EC 2.1.3.3 Enterococcus faecalis ATCC 700802 phosphate + N-carbamoyl-L-ornithine
-
 ?
carbamoyl phosphate + ornithine only weak activity with ornithine as a substrate Enterococcus faecalis phosphate + N-carbamoylornithine
-
 ?
carbamoyl phosphate + putrescine
-
 Enterococcus faecalis phosphate + N-carbamoylputrescine
-
 ?
carbamoyl phosphate + putrescine
-
 Enterococcus faecalis ATCC 700802 phosphate + N-carbamoylputrescine
-
 ?
carbamoyl phosphate + putrescine
-
 Enterococcus faecalis phosphate + N-carbamoyl-putrescine
-
 ?
carbamoyl phosphate + putrescine
-
 Enterococcus faecalis ATCC 700802 phosphate + N-carbamoyl-putrescine
-
 ?
additional information the enzyme catalyzes the second of three steps of ATP synthesis from agmatine Enterococcus faecalis ?
-
 ?
additional information the enzyme catalyzes the second of three steps of ATP synthesis from agmatine Enterococcus faecalis ATCC 700802 ?
-
 ?

Subunits

Subunits Comment Organism
trimer
-
 Enterococcus faecalis
trimer crystal strucutre Enterococcus faecalis

Synonyms

Synonyms Comment Organism
PTC
-
 Enterococcus faecalis
putrescine transcarbamylase
-
 Enterococcus faecalis

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
37
-
 assay at Enterococcus faecalis

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7
-
 assay at Enterococcus faecalis

Ki Value [mM]

Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
0.00001
-
 N-(phosphonoacetyl)-putrescine recombinant enzyme Enterococcus faecalis
10
-
 N-(phosphonoacetyl)-putrescine
-
 Enterococcus faecalis