Literature summary for 2.1.3.2 extracted from

Zhang, P.; Martin, P.D.; Purcarea, C.; Vaishnav, A.; Brunzelle, J.S.; Fernando, R.; Guy-Evans, H.I.; Evans, D.R.; Edwards, B.F.
Dihydroorotase from the hyperthermophile Aquifex aeolicus is activated by stoichiometric association with aspartate transcarbamoylase and forms a one-pot reactor for pyrimidine biosynthesis (2009), Biochemistry, 48, 766-778.

Cloned(Commentary)

Cloned (Comment)	Organism
expression in Escherichia coli strain BL21	Aquifex aeolicus

Crystallization (Commentary)

Crystallization (Comment)	Organism
complex of aspartate transcarbamoylase and dihydroorotase	Aquifex aeolicus
noncovalent hexamer of dihydroorotase and ATCase, to 2.3 A resolution. The structure has citrate, bound to the active sites of both enzymes.Six DHO and six ATC chains form a hollow dodecamer, in which the 12 active sites face an internal reaction chamber that is approximately 60 A in diameter and connected to the cytosol by narrow tunnels. The entrances and the interior of the chamber are both electropositive, which suggests that the architecture of this nanoreactor modifies the kinetics of the bisynthase, not only by steric channeling but also by preferential escape of the product, dihydroorotase	Aquifex aeolicus

Crystallization (Comment)

Organism

complex of aspartate transcarbamoylase and dihydroorotase

Aquifex aeolicus

noncovalent hexamer of dihydroorotase and ATCase, to 2.3 A resolution. The structure has citrate, bound to the active sites of both enzymes.Six DHO and six ATC chains form a hollow dodecamer, in which the 12 active sites face an internal reaction chamber that is approximately 60 A in diameter and connected to the cytosol by narrow tunnels. The entrances and the interior of the chamber are both electropositive, which suggests that the architecture of this nanoreactor modifies the kinetics of the bisynthase, not only by steric channeling but also by preferential escape of the product, dihydroorotase

Aquifex aeolicus

Protein Variants

Protein Variants	Comment	Organism
additional information	in noncovalent association with dihydroorotase, possible model for mammalian polypeptide chain CPSase/ATCase/DHOase during pyrimidine biosynthesis	Aquifex aeolicus

Organism

Organism	UniProt	Comment	Textmining
Aquifex aeolicus	O66726	-	-
Aquifex aeolicus	O66726	in noncovalent association with dihydroorotase, possible model for the mammalian polypeptide chain CPSase/ATCase/DHOase during pyrimidine biosynthesis	-

Subunits

Subunits	Comment	Organism
hexamer	noncovalent association with dihydroorotase	Aquifex aeolicus

Synonyms

Synonyms	Comment	Organism
aspartate transcarbamoylase	-	Aquifex aeolicus