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Literature summary for 2.1.3.2 extracted from

  • Hack, E.S.; Vorobyova, T.; Sakash, J.B.; West, J.M.; Macol, C.P.; Herve, G.; Williams, M.K.; Kantrowitz, E.R.
    Characterization of the aspartate transcarbamoylase from Methanococcus jannaschii (2000), J. Biol. Chem., 275, 15820-15827.
    View publication on PubMed
Search for more literature for 2.1.3.2

Cloned(Commentary)

Cloned (Comment) Organism
-
 Methanocaldococcus jannaschii

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
10.9
-
 L-aspartate at 55°C Methanocaldococcus jannaschii
18.2
-
 L-aspartate at 37°C Methanocaldococcus jannaschii
34.6
-
 L-aspartate at 25°C Methanocaldococcus jannaschii
40.4
-
 L-aspartate at 15°C Methanocaldococcus jannaschii
88
-
 Carbamoyl phosphate at 25°C Methanocaldococcus jannaschii
162
-
 Carbamoylphosphate at 37°C Methanocaldococcus jannaschii

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
17000
-
 x * 37000 + x * 17000, most probably a dodecamer, SDS-PAGE Methanocaldococcus jannaschii
32000
-
 regulatory subunit, gel filtration Methanocaldococcus jannaschii
37000
-
 x * 37000 + x * 17000, most probably a dodecamer, SDS-PAGE Methanocaldococcus jannaschii
129000
-
 catalytic subunit, gelfiltration Methanocaldococcus jannaschii

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
carbamoylphosphate + L-aspartate Escherichia coli
-
 phosphate + N-carbamoyl-L-aspartate
-
 ?
carbamoylphosphate + L-aspartate Methanocaldococcus jannaschii
-
 phosphate + N-carbamoyl-L-aspartate
-
 ?

Organism

Organism UniProt Comment Textmining
Escherichia coli
-
-
-
Methanocaldococcus jannaschii Q58976
-
-

Purification (Commentary)

Purification (Comment) Organism
heat, Q-Sepharose, Phenyl-Sepharose Methanocaldococcus jannaschii

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
carbamoyl phosphate + L-aspartate
-
 Escherichia coli phosphate + N-carbamoyl-L-aspartate
-
 ?
carbamoyl phosphate + L-aspartate
-
 Methanocaldococcus jannaschii phosphate + N-carbamoyl-L-aspartate
-
 ?
carbamoylphosphate + L-aspartate
-
 Escherichia coli phosphate + N-carbamoyl-L-aspartate
-
 ?
carbamoylphosphate + L-aspartate
-
 Methanocaldococcus jannaschii phosphate + N-carbamoyl-L-aspartate
-
 ?

Subunits

Subunits Comment Organism
? x * 37000 + x * 17000, most probably a dodecamer, SDS-PAGE Methanocaldococcus jannaschii

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
75
-
 catalytic subunit looses 25% activity after 60 min Methanocaldococcus jannaschii
75
-
 catalytic subunit looses 50% activity after 1 min Escherichia coli