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Literature summary for 2.1.3.2 extracted from
- Purification and properties of aspartate transcarbamylase from Mycobacterium smegmatis (1988), Biochim. Biophys. Acta, 953, 106-113.
General Stability
| General Stability | Organism |
|---|---|
| carbamoyl phosphate protects against thermal denaturation | Mycolicibacterium smegmatis |
| freezing and thawing in 50 mM Tris-HCl buffer, pH 7.4, 1 mM 2-mercaptoethanol has no effect on stability | Mycolicibacterium smegmatis |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| Ca2+ | - |
Mycolicibacterium smegmatis |  |
| HgSO4 | - |
Mycolicibacterium smegmatis | |
| iodoacetate | - |
Mycolicibacterium smegmatis | |
| Maleate | - |
Mycolicibacterium smegmatis | |
| Mn2+ | - |
Mycolicibacterium smegmatis | |
| Ni2+ | - |
Mycolicibacterium smegmatis | |
| p-hydroxymercuribenzoate | - |
Mycolicibacterium smegmatis | |
| phosphate | - |
Mycolicibacterium smegmatis | |
| succinate | - |
Mycolicibacterium smegmatis | |
| UMP | - |
Mycolicibacterium smegmatis | |
| Zn2+ | - |
Mycolicibacterium smegmatis | |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| additional information | no requirement for any metal ion | Mycolicibacterium smegmatis |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 108000 | - |
1 * 138000 + 1 * 108000, SDS-PAGE | Mycolicibacterium smegmatis |
| 138000 | - |
1 * 138000 + 1 * 108000, SDS-PAGE | Mycolicibacterium smegmatis |
| 246000 | - |
gel filtration | Mycolicibacterium smegmatis |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| carbamoylphosphate + L-aspartate | Mycolicibacterium smegmatis | - |
phosphate + N-carbamoyl-L-aspartate | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Mycolicibacterium smegmatis | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| streptomycin sulfate, ammonium sulfate, DE-52 column, Sephadex G-200, aspartate linked CNBr | Mycolicibacterium smegmatis |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 7900 | - |
- |
Mycolicibacterium smegmatis |
Storage Stability
| Storage Stability | Organism |
|---|---|
| -20°C, 2 mM carbamoyl phosphate, 1 mM 2-mercaptoethanol, at least a week, no loss of activity | Mycolicibacterium smegmatis |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| carbamoyl phosphate + L-aspartate | - |
Mycolicibacterium smegmatis | phosphate + N-carbamoyl-L-aspartate | - |
? | |
| carbamoylphosphate + L-aspartate | - |
Mycolicibacterium smegmatis | phosphate + N-carbamoyl-L-aspartate | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| dimer | 1 * 138000 + 1 * 108000, SDS-PAGE | Mycolicibacterium smegmatis |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 45 | - |
loss of activity after 10 min, carbamoylphosphate protects | Mycolicibacterium smegmatis |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7.8 | - |
- |
Mycolicibacterium smegmatis |
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