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Literature summary for 2.1.3.2 extracted from
- Aspartate transcarbamylase from Bacillus subtilis (1985), Methods Enzymol., 113, 627-635.
Crystallization (Commentary)
| Crystallization (Comment) | Organism |
|---|---|
| 50 mM Tis-HCl, pH 8.1, 70% ammonium sulfate | Bacillus subtilis |
General Stability
| General Stability | Organism |
|---|---|
| carbamoyl phosphate protects against proteolysis | Bacillus subtilis |
| carboxylic acids, e.g. acetic acid, citric acid and high ionic strength, 200 mM KCl, protect against thermal denaturation at 65-70°C | Bacillus subtilis |
| unstable in very dilute solutions | Bacillus subtilis |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| N-(Phosphonoacetyl)-L-aspartate | - |
Bacillus subtilis |  |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.11 | - |
Carbamoyl phosphate | - |
Bacillus subtilis | |
| 7 | - |
L-aspartate | - |
Bacillus subtilis | |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| carbamoylphosphate + L-aspartate | Bacillus subtilis | - |
phosphate + N-carbamoyl-L-aspartate | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Bacillus subtilis | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| protamine sulfate, heat treatment, ammonium sulfate, Sephadex G-150, DEAE-Sephadex, DEAE-cellulose | Bacillus subtilis |
Reaction
| Reaction | Comment | Organism | Reaction ID |
|---|---|---|---|
| carbamoyl phosphate + L-aspartate = phosphate + N-carbamoyl-L-aspartate | reaction mechanism | Bacillus subtilis |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 560 | - |
- |
Bacillus subtilis |
Storage Stability
| Storage Stability | Organism |
|---|---|
| -10°C -20°C, many months, no loss of activity | Bacillus subtilis |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| carbamoyl phosphate + L-aspartate | - |
Bacillus subtilis | phosphate + N-carbamoyl-L-aspartate | - |
? | |
| carbamoylphosphate + L-aspartate | - |
Bacillus subtilis | phosphate + N-carbamoyl-L-aspartate | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| trimer | - |
Bacillus subtilis |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| additional information | - |
carboxylic acids e.g. acetic acid, citric acid and high ionic strength, 200 mM KCl, protect against thermal denaturation at 65-70°C | Bacillus subtilis |
- |
25 | quite stable | Bacillus subtilis |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 8.5 | - |
- |
Bacillus subtilis |
pH Stability
| pH Stability | pH Stability Maximum | Comment | Organism |
|---|---|---|---|
| 6 | - |
unstable below | Bacillus subtilis |
Ki Value [mM]
| Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.000006 | - |
N-(Phosphonoacetyl)-L-aspartate | - |
Bacillus subtilis | |
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