General Stability | Organism |
---|---|
dimethylsulfoxid and glycerol are required to stabilize the enzyme | Mesocricetus auratus |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
aspartate | at high concentrations | Mesocricetus auratus |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.009 | - |
L-aspartate | multienzyme complex | Mesocricetus auratus | |
0.02 | - |
Carbamoyl phosphate | multienzyme complex and aspartate transcarbamoylase activity domain of the multienzyme complex alone | Mesocricetus auratus | |
0.0207 | - |
Carbamoyl phosphate | - |
Mesocricetus auratus | |
0.021 | - |
L-aspartate | aspartate transcarbamoylase activity domain of the multienzyme complex alone | Mesocricetus auratus | |
0.02134 | - |
L-aspartate | - |
Mesocricetus auratus |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
40000 | - |
hamster aspartate carbamoyltransferase domain is an oligomer consisting of 2 or 3 identical copies of the 40000 Da proteolytic fragment of CAD, 2 or 3 * 40000, SDS-PAGE | Mesocricetus auratus |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
carbamoylphosphate + L-aspartate | Mesocricetus auratus | - |
phosphate + N-carbamoyl-L-aspartate | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Mesocricetus auratus | - |
- |
- |
Purification (Comment) | Organism |
---|---|
aspartate transcarbamoylase activity domain of the multienzyme complex | Mesocricetus auratus |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
cell culture | simian virus 40 transformed syrian hamster cell line | Mesocricetus auratus | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
carbamoyl phosphate + L-aspartate | - |
Mesocricetus auratus | phosphate + N-carbamoyl-L-aspartate | - |
? | |
carbamoylphosphate + L-aspartate | - |
Mesocricetus auratus | phosphate + N-carbamoyl-L-aspartate | - |
? |
Subunits | Comment | Organism |
---|---|---|
More | hamster aspartate carbamoyltransferase domain is an oligomer consisting of 2 or 3 identical copies of the 40000 Da proteolytic fragment of CAD, 2 or 3 * 40000, SDS-PAGE | Mesocricetus auratus |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
35.9 | - |
Carbamoyl phosphate | aspartate transcarbamoylase activity domain of the multienzyme complex | Mesocricetus auratus | |
57.4 | - |
Carbamoylphosphate | multienzyme complex | Mesocricetus auratus | |
79.7 | - |
aspartate | aspartate transcarbamoylase activity domain of the multienzyme complex | Mesocricetus auratus | |
156 | - |
L-aspartate | multienzyme complex | Mesocricetus auratus |
Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
---|---|---|---|---|---|
4.59 | - |
L-aspartate | aspartate transcarbamoylase activity domain of the multienzyme complex | Mesocricetus auratus | |
13.56 | - |
L-aspartate | multienzyme complex | Mesocricetus auratus |