Cloned (Comment) | Organism |
---|---|
expression of N-terminally His6-tagged MtbATIC in Escherichia coli strain BL21(DE3) | Mycobacterium tuberculosis |
Crystallization (Comment) | Organism |
---|---|
purified recombinant His-tagged enzyme free or in complex with substrate 5-aminoimidazole-4-carboxamide ribonucleotide, both with adventitiously active site-bound nucleotide inhibitor of the cyclohydrolase activity, X-ray diffraction structure determination and analysis at 2.5 A and 2.2 A resolution, respectively | Mycobacterium tuberculosis |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
additional information | an adventitiously active site-bound nucleotide, found in the crystal structures, is identified by NMR and mass spectral analysis as a novel 5-formyl derivative of an earlier intermediate in the biosynthetic pathway 4-carboxy-5-aminoimidazole ribonucleotide. The nucleotide is a cyclohydrolase inhibitor. Binding structure analysis, structure modeling, overview | Mycobacterium tuberculosis |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Mycobacterium tuberculosis | - |
gene Rv0957 | - |
Mycobacterium tuberculosis H37Rv | - |
gene Rv0957 | - |
Purification (Comment) | Organism |
---|---|
recombinant N-terminally His6-tagged MtbATIC in Escherichia coli strain BL21(DE3) by nickel affinity chromatography | Mycobacterium tuberculosis |
Subunits | Comment | Organism |
---|---|---|
More | the protein is folded into two domains, the N-terminal domain, residues 1-212, containing the cyclohydrolase active site and the C-terminal domain, residues 222-523, containing the formyltransferase active site, structure modeling, overview | Mycobacterium tuberculosis |
Synonyms | Comment | Organism |
---|---|---|
5-aminoimidazole-4-carboxamide ribonucleotide transformylase | - |
Mycobacterium tuberculosis |
5-aminoimidazole-4-carboxamide ribonucleotide transformylase/inosine monophosphate cyclohydrolase | - |
Mycobacterium tuberculosis |
AICAR formyltransferase | - |
Mycobacterium tuberculosis |
ATIC | - |
Mycobacterium tuberculosis |
PurH | - |
Mycobacterium tuberculosis |
General Information | Comment | Organism |
---|---|---|
additional information | the enzyme is part of the the bifunctional enzyme 5-aminoimidazole-4-carboxamide ribonucleotide transformylase/inosine monophosphate cyclohydrolase, ATIC, or PurH | Mycobacterium tuberculosis |