Literature summary for 2.1.2.3 extracted from

Le Nours, J.; Bulloch, E.M.; Zhang, Z.; Greenwood, D.R.; Middleditch, M.J.; Dickson, J.M.; Baker, E.N.
Structural analyses of a purine biosynthetic enzyme from Mycobacterium tuberculosis reveal a novel bound nucleotide (2011), J. Biol. Chem., 286, 40706-40716.

Search for more literature for 2.1.2.3

Cloned(Commentary)

Cloned (Comment)	Organism
expression of N-terminally His6-tagged MtbATIC in Escherichia coli strain BL21(DE3)	Mycobacterium tuberculosis

Crystallization (Commentary)

Crystallization (Comment)	Organism
purified recombinant His-tagged enzyme free or in complex with substrate 5-aminoimidazole-4-carboxamide ribonucleotide, both with adventitiously active site-bound nucleotide inhibitor of the cyclohydrolase activity, X-ray diffraction structure determination and analysis at 2.5 A and 2.2 A resolution, respectively	Mycobacterium tuberculosis

Inhibitors

Inhibitors	Comment	Organism	Structure
additional information	an adventitiously active site-bound nucleotide, found in the crystal structures, is identified by NMR and mass spectral analysis as a novel 5-formyl derivative of an earlier intermediate in the biosynthetic pathway 4-carboxy-5-aminoimidazole ribonucleotide. The nucleotide is a cyclohydrolase inhibitor. Binding structure analysis, structure modeling, overview	Mycobacterium tuberculosis

Organism

Organism	UniProt	Comment	Textmining
Mycobacterium tuberculosis	-	gene Rv0957	-
Mycobacterium tuberculosis H37Rv	-	gene Rv0957	-

Purification (Commentary)

Purification (Comment)	Organism
recombinant N-terminally His6-tagged MtbATIC in Escherichia coli strain BL21(DE3) by nickel affinity chromatography	Mycobacterium tuberculosis

Subunits

Subunits	Comment	Organism
More	the protein is folded into two domains, the N-terminal domain, residues 1-212, containing the cyclohydrolase active site and the C-terminal domain, residues 222-523, containing the formyltransferase active site, structure modeling, overview	Mycobacterium tuberculosis

Synonyms

Synonyms	Comment	Organism
5-aminoimidazole-4-carboxamide ribonucleotide transformylase	-	Mycobacterium tuberculosis
5-aminoimidazole-4-carboxamide ribonucleotide transformylase/inosine monophosphate cyclohydrolase	-	Mycobacterium tuberculosis
AICAR formyltransferase	-	Mycobacterium tuberculosis
ATIC	-	Mycobacterium tuberculosis
PurH	-	Mycobacterium tuberculosis

General Information

General Information	Comment	Organism
additional information	the enzyme is part of the the bifunctional enzyme 5-aminoimidazole-4-carboxamide ribonucleotide transformylase/inosine monophosphate cyclohydrolase, ATIC, or PurH	Mycobacterium tuberculosis

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Release 2023.1 (January 2023)