Literature summary for 2.1.2.2 extracted from

Zhai, F.; Liu, X.; Ruan, J.; Li, J.; Liu, Z.; Hu, Y.; Li, S.
Expression, crystallization and preliminary X-ray analysis of the phosphoribosylglycinamide formyltransferase from Streptococcus mutans (2011), Acta Crystallogr. Sect. F, 67, 287-290.

Cloned(Commentary)

Cloned (Comment)	Organism
recombinant expression of His-tagged enzyme in Escherichia coli strain BL21 (DE3)	Streptococcus mutans

Crystallization (Commentary)

Crystallization (Comment)	Organism
purified recombinant enzyme, hanging drop vapour diffusion method, mixing of 0.001 ml of 22 mg/ml protein solution with 0.001 ml of reservoir solution containing 0.2 M lithium chloride, 0.1 M sodium cacodylate trihydrate, pH 6.6, 14% PEG 3350, and equilibration against 0.2 ml of reservoir solution, 18°C, method optimization, X-ray diffraction structure determination and analysis at 2.1 A resolution, molecular replacement	Streptococcus mutans

Crystallization (Comment)

Organism

purified recombinant enzyme, hanging drop vapour diffusion method, mixing of 0.001 ml of 22 mg/ml protein solution with 0.001 ml of reservoir solution containing 0.2 M lithium chloride, 0.1 M sodium cacodylate trihydrate, pH 6.6, 14% PEG 3350, and equilibration against 0.2 ml of reservoir solution, 18°C, method optimization, X-ray diffraction structure determination and analysis at 2.1 A resolution, molecular replacement

Streptococcus mutans

Organism

Organism	UniProt	Comment	Textmining
Streptococcus mutans	-	-	-

Purification (Commentary)

Purification (Comment)	Organism
recombinant His-tagged enzyme from Escherichia coli strain BL21 (DE3) by nickel affinity and anion exchange chromatography, and gel filtration	Streptococcus mutans

Synonyms

Synonyms	Comment	Organism
PurN	-	Streptococcus mutans

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Release 2023.1 (January 2023)