Literature summary for 2.1.2.2 extracted from

Nixon, A.E.; Benkovic, S.J.
Improvement in the efficiency of formyl transfer of a GAR transformylase hybrid enzyme (2000), Protein Eng., 13, 323-327.

Search for more literature for 2.1.2.2

Cloned(Commentary)

Cloned (Comment)	Organism
hybrid glycinamide ribonucleotide transformylase, ribonucleotide binding domain from purN, catalytic machinery and formyl tetrahydrofolate binding domain from purU encoded formyl tetrahydrofolate hydrolase	Escherichia coli

KM Value [mM]

KM Value [mM]	KM Value Maximum [mM]	Substrate	Comment	Organism	Structure
0.016	-	beta-glycinamide ribonucleotide	purN/purU hybrid enzyme	Escherichia coli
0.017	-	10-formyl-5,8-dideazafolate	-	Escherichia coli
0.019	-	beta-glycinamide ribonucleotide	-	Escherichia coli
0.035	-	10-formyl-5,8-dideazafolate	purN/purU hybrid enzyme	Escherichia coli

Molecular Weight [Da]

Molecular Weight [Da]	Molecular Weight Maximum [Da]	Comment	Organism
23000	-	purN encoded, DNA sequencing	Escherichia coli

Organism

Organism	UniProt	Comment	Textmining
Escherichia coli	-	strains DH5alpha, BL21(DE3)	-

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
10-formyltetrahydrofolate + 5-phospho-D-ribosylglycinamide	-	Escherichia coli	tetrahydrofolate + 5'-phosphoribosyl-N-formylglycinamide	-	?

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Release 2023.1 (January 2023)