Inhibitors | Comment | Organism | Structure |
---|---|---|---|
3-Methyl-2-butanone | 5 mM, 27% inhibition | Escherichia coli | |
coenzyme A | above 1 mM | Escherichia coli | |
D-valine | 5 mM, 16% inhibition | Escherichia coli | |
formaldehyde | 0.8 mM, partial inhibition | Escherichia coli | |
Isovalerate | 5 mM, 39% inhibition | Escherichia coli | |
L-valine | 5 mM, 23% inhibition | Escherichia coli | |
additional information | not inactivated by borohydride reduction in the presence of excess substrates | Escherichia coli | |
Pantoate | 0.05 mM or above | Escherichia coli | |
pantothenate | 0.5 mM or above | Escherichia coli | |
pyruvate | 5 mM, 38% inhibition | Escherichia coli | |
tetrahydrofolate | 0.38 mM, partial inhibition | Escherichia coli |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.1 | - |
Tetrahydropteroylpentaglutamate | - |
Escherichia coli | |
0.1 | - |
tetrahydropteroyltetraglutamate | - |
Escherichia coli | |
0.16 | - |
ketopantoate | - |
Escherichia coli | |
0.17 | - |
tetrahydropteroylhexaglutamate | - |
Escherichia coli | |
0.18 | - |
tetrahydropteroyltriglutamate | - |
Escherichia coli | |
0.18 | - |
tetrahydrofolate | forward and reverse reaction, methylenetetrahydrofolate: formaldehyde + tetrahydrofolate | Escherichia coli | |
0.25 | - |
tetrahydropteroyldiglutamate | - |
Escherichia coli | |
0.29 | - |
tetrahydropteroylheptaglutamate | - |
Escherichia coli | |
0.33 | - |
tetrahydropteroylmonoglutamate | - |
Escherichia coli | |
1.1 | - |
3-methyl-2-oxobutanoate | - |
Escherichia coli | |
2.9 | - |
2-oxobutyrate | - |
Escherichia coli | |
5.9 | - |
3-methyl-2-oxopentanoate | - |
Escherichia coli | |
5.9 | - |
formaldehyde | methylenetetrahydrofolate: formaldehyde + tetrahydrofolate | Escherichia coli | |
25 | - |
2-oxopentanoate | - |
Escherichia coli |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Co2+ | activates, Mg2+ is most active followed by Mn2+, Ni2+, Co2+ and Zn2+ | Escherichia coli | |
Mg2+ | activates and is required for activity, 0.1 mM Mg2+ is most active, Mn2+, Ni2+, Co2+ and Zn2+ are progressively less active, restores activity after dialysis | Escherichia coli | |
Mn2+ | activates, Mg2+ is most active followed by Mn2+, Ni2+, Co2+ and Zn2+ | Escherichia coli | |
additional information | not activated by Cu2+ and Fe2+ | Escherichia coli | |
Ni2+ | activates, Mg2+ is most active followed by Mn2+, Ni2+, Co2+ and Zn2+ | Escherichia coli | |
Zn2+ | activates, Mg2+ is most active followed by Mn2+, Ni2+, Co2+ and Zn2+ | Escherichia coli |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
25700 | - |
10 * 27000, SDS-PAGE, 10 * 25700, amino acid analysis | Escherichia coli |
27000 | - |
10 * 27000, SDS-PAGE, 10 * 25700, amino acid analysis | Escherichia coli |
255000 | - |
sedimentation equilibrium | Escherichia coli |
285000 | - |
gel filtration | Escherichia coli |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
5,10-methylenetetrahydrofolate + 3-methyl-2-oxobutanoate | Escherichia coli | first committed step in pantothenate biosynthesis | tetrahydrofolate + 2-dehydropantoate | - |
? | |
5,10-methylenetetrahydrofolate + 3-methyl-2-oxobutanoate | Escherichia coli | catalytic activity is regulated by the products of the reaction path of which it is one component | tetrahydrofolate + 2-dehydropantoate | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Escherichia coli | - |
- |
- |
Purification (Comment) | Organism |
---|---|
- |
Escherichia coli |
Reaction | Comment | Organism | Reaction ID |
---|---|---|---|
5,10-methylenetetrahydrofolate + 3-methyl-2-oxobutanoate + H2O = tetrahydrofolate + 2-dehydropantoate | class II aldolase | Escherichia coli |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
5,10-methylenetetrahydrofolate + 2-oxobutyrate | also a good substrate | Escherichia coli | ? | - |
? | |
5,10-methylenetetrahydrofolate + 2-oxopentanoate | - |
Escherichia coli | ? | - |
? | |
5,10-methylenetetrahydrofolate + 3-methyl-2-oxobutanoate | specificity for alpha-ketoisovalerate is less rigid than for tetrahydrofolate | Escherichia coli | tetrahydrofolate + 2-dehydropantoate | synthesis of ketopantoate, the following components can replace tetrahydrofolate: tetrahydropteroylmono-, di-, tri-, tetra-, penta-, hexa-, and heptaglutamate, absolute requirement for tetrahydrofolate, only the L-isomer is active | r | |
5,10-methylenetetrahydrofolate + 3-methyl-2-oxobutanoate | first committed step in pantothenate biosynthesis | Escherichia coli | tetrahydrofolate + 2-dehydropantoate | - |
? | |
5,10-methylenetetrahydrofolate + 3-methyl-2-oxobutanoate | catalytic activity is regulated by the products of the reaction path of which it is one component | Escherichia coli | tetrahydrofolate + 2-dehydropantoate | - |
? | |
5,10-methylenetetrahydrofolate + 3-methyl-2-oxopentanoate | - |
Escherichia coli | ? | - |
? | |
formaldehyde + tetrahydrofolate | - |
Escherichia coli | methylentetrahydrofolate | - |
? | |
additional information | no substrates: pyruvate, isovalerate, D- and L-valine, 3-methyl-2-butanone | Escherichia coli | ? | - |
? | |
tetrahydropteroyldiglutamate + 2-dehydropantoate | - |
Escherichia coli | 5,10-methylenetetrahydropteroyldiglutamate + 3-methyl-2-oxobutanoate + H2O | - |
r | |
tetrahydropteroylheptaglutamate + 2-dehydropantoate | - |
Escherichia coli | 5,10-methylenetetrahydropteroylheptaglutamate + 3-methyl-2-oxobutanoate + H2O | - |
r | |
tetrahydropteroylhexaglutamate + 2-dehydropantoate | - |
Escherichia coli | 5,10-methylenetetrahydropteroylhexaglutamate + 3-methyl-2-oxobutanoate + H2O | - |
r | |
tetrahydropteroylpentaglutamate + 2-dehydropantoate | - |
Escherichia coli | 5,10-methylenetetrahydropteroylpentaglutamate + 3-methyl-2-oxobutanoate + H2O | - |
r | |
tetrahydropteroyltetraglutamate + 2-dehydropantoate | - |
Escherichia coli | 5,10-methylenetetrahydropteroyltetraglutamate + 3-methyl-2-oxobutanoate + H2O | - |
r | |
tetrahydropteroyltriglutamate + 2-dehydropantoate | - |
Escherichia coli | 5,10-methylenetetrahydropteroyltriglutamate + 3-methyl-2-oxobutanoate + H2O | - |
r |
Subunits | Comment | Organism |
---|---|---|
decamer | 10 * 27000, SDS-PAGE, 10 * 25700, amino acid analysis | Escherichia coli |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
70 | 80 | reverse reaction, activity decreases rapidly above 80°C | Escherichia coli |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
80 | - |
rapid denaturation above | Escherichia coli |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
additional information | - |
pI: 4.4 | Escherichia coli |
7 | 7.6 | - |
Escherichia coli |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
5 | 9 | about 50% of activity maximum at pH 6 and 9, inactive below pH 5 | Escherichia coli |
pH Stability | pH Stability Maximum | Comment | Organism |
---|---|---|---|
4.5 | - |
rapid inactivation below | Escherichia coli |
5 | 10 | stable | Escherichia coli |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
5,10-methylenetetrahydrofolate | - |
Escherichia coli | |
tetrahydrofolate | absolute requirement for tetrahydrofolate | Escherichia coli |