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Literature summary for 2.1.2.1 extracted from
- A serine hydroxymethyltransferase from marine bacterium Shewanella algae: Isolation, purification, characterization and l-serine production (2013), Microbiol. Res., 168, 477-484.
Activating Compound
| Activating Compound | Comment | Organism | Structure |
|---|---|---|---|
| CO(NH2)2 | activates | Shewanella algae |  |
| EDTA | activates | Shewanella algae | |
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| gene glyA, DNA and amino acid sequence determination and analysis, phylogenetic analysis and tree, subcloning in Escherichia coli strain DH5alpha, recombinant expression of GST-tagged enzyme in Escherichia coli strain BL21(DE3) | Shewanella algae |
Protein Variants
| Protein Variants | Comment | Organism |
|---|---|---|
| additional information | the SaSHMT has the potential for industrial applications due to its tolerance of alkaline environment and a relatively high enzymatic conversion rate | Shewanella algae |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| Co2+ | slight inhibition; strong inhibition | Shewanella algae | |
| CTAB | strong inhibition | Shewanella algae | |
| DTT | strong inhibition | Shewanella algae | |
| Fe2+ | slight inhibition | Shewanella algae | |
| Hg2+ | strong inhibition | Shewanella algae | |
| SDS | strong inhibition | Shewanella algae | |
| Zn2+ | strong inhibition | Shewanella algae | |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Ba2+ | activates | Shewanella algae | |
| Ca2+ | activates | Shewanella algae | |
| K+ | activates | Shewanella algae | |
| Mg2+ | activates | Shewanella algae | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 45300 | - |
x * 45300, recombinant GST-tagged enzyme, SDS-PAGE | Shewanella algae |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 5,10-methylenetetrahydrofolate + glycine + H2O | Shewanella algae | - |
tetrahydrofolate + L-serine | - |
r | |
| additional information | Shewanella algae | the enzyme shows high serine hydroxymethyltransferase activity and L-serine production | ? | - |
? | |
| tetrahydrofolate + L-serine | Shewanella algae | - |
5,10-methylenetetrahydrofolate + glycine + H2O | - |
r | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Shewanella algae | R9YJZ9 | gene glyA | - |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| recombinant GST-tagged enzyme from Escherichia coli strain Bl21(DE3) by glutathione affinity chromatography | Shewanella algae |
Storage Stability
| Storage Stability | Organism |
|---|---|
| 4°C, purified recombinant enzyme, 96 h, over 60% of maximal activity remains | Shewanella algae |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 5,10-methylenetetrahydrofolate + glycine + H2O | - |
Shewanella algae | tetrahydrofolate + L-serine | - |
r | |
| additional information | the enzyme shows high serine hydroxymethyltransferase activity and L-serine production | Shewanella algae | ? | - |
? | |
| tetrahydrofolate + L-serine | - |
Shewanella algae | 5,10-methylenetetrahydrofolate + glycine + H2O | - |
r | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| ? | x * 45300, recombinant GST-tagged enzyme, SDS-PAGE | Shewanella algae |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| serine hydroxymethyltransferase | - |
Shewanella algae |
| SHMT | - |
Shewanella algae |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 34 | - |
- |
Shewanella algae |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 40 | - |
the enzyme retains over 45% of its maximal activity after incubation at 40°C for 3 h | Shewanella algae |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7 | - |
- |
Shewanella algae |
pH Range
| pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|
| 5.5 | 9.5 | maximal activity at pH 7.0, 88% of the maximal activity at pH 7.0-8.0, less than 20% of maximal activity at pH 9.5, nearly no activity below pH 5.5 | Shewanella algae |
pH Stability
| pH Stability | pH Stability Maximum | Comment | Organism |
|---|---|---|---|
| 5.5 | 9 | purified recombinant SaSHMT shows significant stability under weakly acidic and alkaline environment at pH 6.5-9.0. Without any stabilizer at pH 6.5-9.0 for 96 h at 4°C over 80% of maximum activity is retained, at pH 5.5-6.0 less than 80% activity remains, the enzyme is unstable below pH 5.5 | Shewanella algae |
Cofactor
| Cofactor | Comment | Organism | Structure |
|---|---|---|---|
| 5,10-methylenetetrahydrofolate | - |
Shewanella algae | |
| pyridoxal 5'-phosphate | - |
Shewanella algae | |
| tetrahydrofolate | - |
Shewanella algae | |
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