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Literature summary for 2.1.2.1 extracted from

  • Angelaccio, S.; Di Salvo, M.; Parroni, A.; Di Bello, A.; Contestabile, R.; Pascarella, S.
    Structural stability of cold-adapted serine hydroxymethyltransferase, a tool for beta-hydroxy-alpha-amino acid biosynthesis (2014), J. Mol. Catal. B, 110, 171-177.
No PubMed abstract available

Inhibitors

Inhibitors Comment Organism Structure
formaldehyde inactivation Escherichia coli
formaldehyde inactivation Psychromonas ingrahamii

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
additional information
-
additional information steady-state kinetics Escherichia coli
additional information
-
additional information steady-state kinetics Psychromonas ingrahamii
0.3
-
L-serine pH 7.2, 20°C Escherichia coli
0.4
-
L-serine pH 7.2, 20°C Psychromonas ingrahamii

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
5,10-methylenetetrahydrofolate + glycine + H2O Escherichia coli
-
tetrahydrofolate + L-serine
-
r
5,10-methylenetetrahydrofolate + glycine + H2O Psychromonas ingrahamii
-
tetrahydrofolate + L-serine
-
r
5,10-methylenetetrahydrofolate + glycine + H2O Psychromonas ingrahamii 37
-
tetrahydrofolate + L-serine
-
r
additional information Psychromonas ingrahamii SHMT catalyses the reversible cleavage of several beta-hydroxy amino acids varying in substituent and stereochemistry at Cbeta. The enzyme also has L-threo-beta-phenylserine aldolase activity ?
-
?
additional information Psychromonas ingrahamii 37 SHMT catalyses the reversible cleavage of several beta-hydroxy amino acids varying in substituent and stereochemistry at Cbeta. The enzyme also has L-threo-beta-phenylserine aldolase activity ?
-
?
tetrahydrofolate + L-serine Escherichia coli
-
5,10-methylenetetrahydrofolate + glycine + H2O
-
r
tetrahydrofolate + L-serine Psychromonas ingrahamii
-
5,10-methylenetetrahydrofolate + glycine + H2O
-
r
tetrahydrofolate + L-serine Psychromonas ingrahamii 37
-
5,10-methylenetetrahydrofolate + glycine + H2O
-
r

Organism

Organism UniProt Comment Textmining
Escherichia coli P0A825 gene glyA
-
Psychromonas ingrahamii A1SUU0 gene glyA
-
Psychromonas ingrahamii 37 A1SUU0 gene glyA
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
5,10-methylenetetrahydrofolate + glycine + H2O
-
Escherichia coli tetrahydrofolate + L-serine
-
r
5,10-methylenetetrahydrofolate + glycine + H2O
-
Psychromonas ingrahamii tetrahydrofolate + L-serine
-
r
5,10-methylenetetrahydrofolate + glycine + H2O
-
Psychromonas ingrahamii 37 tetrahydrofolate + L-serine
-
r
additional information SHMT catalyses the reversible cleavage of several beta-hydroxy amino acids varying in substituent and stereochemistry at Cbeta. The enzyme also has L-threo-beta-phenylserine aldolase activity Psychromonas ingrahamii ?
-
?
additional information SHMT catalyses the reversible cleavage of several beta-hydroxy amino acids varying in substituent and stereochemistry at Cbeta. The enzyme also has L-threo-beta-phenylserine aldolase activity Psychromonas ingrahamii 37 ?
-
?
tetrahydrofolate + L-serine
-
Escherichia coli 5,10-methylenetetrahydrofolate + glycine + H2O
-
r
tetrahydrofolate + L-serine
-
Psychromonas ingrahamii 5,10-methylenetetrahydrofolate + glycine + H2O
-
r
tetrahydrofolate + L-serine
-
Psychromonas ingrahamii 37 5,10-methylenetetrahydrofolate + glycine + H2O
-
r

Synonyms

Synonyms Comment Organism
serine hydroxymethyltransferase
-
Escherichia coli
serine hydroxymethyltransferase
-
Psychromonas ingrahamii
SHMT
-
Escherichia coli
SHMT
-
Psychromonas ingrahamii

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
20
-
assay at Escherichia coli
20
-
assay at Psychromonas ingrahamii

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
9.25
-
L-serine pH 7.2, 20°C Psychromonas ingrahamii
13.17
-
L-serine pH 7.2, 20°C Escherichia coli

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.2
-
assay at Escherichia coli
7.2
-
assay at Psychromonas ingrahamii

Cofactor

Cofactor Comment Organism Structure
5,10-methylenetetrahydrofolate
-
Escherichia coli
5,10-methylenetetrahydrofolate
-
Psychromonas ingrahamii
pyridoxal 5'-phosphate dependent on Escherichia coli
pyridoxal 5'-phosphate dependent on Psychromonas ingrahamii
tetrahydrofolate
-
Escherichia coli
tetrahydrofolate
-
Psychromonas ingrahamii

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
23.13
-
L-serine pH 7.2, 20°C Psychromonas ingrahamii
43.88
-
L-serine pH 7.2, 20°C Escherichia coli