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Literature summary for 2.1.2.1 extracted from
- Molecular cloning and biochemical characterization of Leishmania donovani serine hydroxymethyltransferase (2007), Protein Expr. Purif., 52, 433-440.
Application
| Application | Comment | Organism |
|---|---|---|
| medicine | drug target in Leishmania | Leishmania donovani |
| medicine | ldSHMT is of medical importance as a drug target since ldSHMT is preferentially expressed in the amastigote stage of parasite which resides in human macrophages | Leishmania donovani |
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| expressed as a His-tagged fusion protein in Escherichia coli | Leishmania donovani |
| into pQE60 vector having C terminal His6-tag, expression in Escherichia coli M15 cells | Leishmania donovani |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| guanidine hydrochloride | 86% loss of activity at 0.25 M | Leishmania donovani |  |
| tetrahydrofolate | inhibitory at non-saturating concentration of serine, at concentrations above 1.5 mM | Leishmania donovani | |
| Urea | 81% loss of activity at 1M | Leishmania donovani | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 1.6 | - |
L-serine | - |
Leishmania donovani | |
| 1.6 | - |
serine | - |
Leishmania donovani | |
| 2.4 | - |
tetrahydrofolate | - |
Leishmania donovani | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 54000 | - |
SDS-PAGE | Leishmania donovani |
| 216000 | - |
gel filtration | Leishmania donovani |
| 216000 | - |
gel filtration, tetramer | Leishmania donovani |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Leishmania donovani | Q86LS9 | - |
- |
| Leishmania donovani MHOM/IN/80/Dd8 | Q86LS9 | - |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| by Ni2+-NTA affinity chromatography, 95% pure | Leishmania donovani |
| using Ni-NTA chromatography | Leishmania donovani |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| amastigote | - |
Leishmania donovani | - |
| additional information | gene is preferentially expressed in the amastigote stage of parasite | Leishmania donovani | - |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 1.79 | - |
- |
Leishmania donovani |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 5,10-methylenetetrahydrofolate + glycine + H2O | - |
Leishmania donovani | tetrahydrofolate + L-serine | - |
r | |
| 5,10-methylenetetrahydrofolate + glycine + H2O | - |
Leishmania donovani MHOM/IN/80/Dd8 | tetrahydrofolate + L-serine | - |
r | |
| L-serine + tetrahydrofolate | - |
Leishmania donovani | glycine + 5,10-methylenetetrahydrofolate + H2O | - |
? | |
| L-serine + tetrahydrofolate | - |
Leishmania donovani MHOM/IN/80/Dd8 | glycine + 5,10-methylenetetrahydrofolate + H2O | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| tetramer | 4 * 54000, SDS-PAGE, gel filtration | Leishmania donovani |
| tetramer | 4 * 54000 Da, gel filtration | Leishmania donovani |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| serine hydroxymethyltransferase | - |
Leishmania donovani |
| SHMT | - |
Leishmania donovani |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 37 | - |
assay at | Leishmania donovani |
| 40 | - |
- |
Leishmania donovani |
Temperature Range [°C]
| Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
|---|---|---|---|
| additional information | - |
enzyme is thermally stable up to 45°C where it exhibits 85% activity. At 65°C it loses its activity | Leishmania donovani |
| 38 | 60 | - |
Leishmania donovani |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| additional information | - |
stable up to 45°C | Leishmania donovani |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7.8 | - |
- |
Leishmania donovani |
| 9 | - |
enzyme retains more than 50% activity at pH 9.0 | Leishmania donovani |
pH Range
| pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|
| 6 | 9 | - |
Leishmania donovani |
pI Value
| Organism | Comment | pI Value Maximum | pI Value |
|---|---|---|---|
| Leishmania donovani | - |
- |
8.9 |
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