Cloned (Comment) | Organism |
---|---|
wild-type and mutant enzymes S52A, S52C and R262A are overexpressed in Escherichia coli | Ovis aries |
Protein Variants | Comment | Organism |
---|---|---|
R262A | KM-value for L-Ser is 5.2fold higher than the wild-type value, the turnover-number is 5.25fold lower than the wild-type value, no transamination reaction with D-Ala, reaction with L-allo-Thr is 20fold decreased compared to wild-type value. L-Ser is unable to enhance the thermal stability as it does in wild-type enzyme | Ovis aries |
S52A | KM-value for L-Ser is 1.3fold higher than the wild-type value, the turnover-number is 6fold lower than the wild-type value, transamination reaction with D-Ala is 20% of the wild-type activity, reaction with L-allo-Thr is 15% of the wild-type activity. L-Ser is unable to enhance the thermal stability as it does in wild-type enzyme | Ovis aries |
S52C | KM-value for L-Ser is 11.2fold higher than the wild-type value, the turnover-number is 21fold lower than the wild-type value, no transamination reaction with D-Ala, no reaction with L-allo-Thr. L-Ser is unable to enhance the thermal stability as it does in wild-type enzyme | Ovis aries |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
1 | - |
L-Ser | pH 7.4, 37°C, wild-type enzyme | Ovis aries | |
1.3 | - |
L-Ser | pH 7.4, 37°C, mutant enzyme S52A | Ovis aries | |
5.2 | - |
L-Ser | pH 7.4, 37°C, mutant enzyme R262A | Ovis aries | |
11.2 | - |
L-Ser | pH 7.4, 37°C, mutant enzyme S52C | Ovis aries |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
cytosol | - |
Ovis aries | 5829 | - |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
53000 | - |
4 * 53000, SDS-PAGE | Ovis aries |
210000 | - |
gel filtration | Ovis aries |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Ovis aries | - |
- |
- |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
liver | - |
Ovis aries | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
5,6,7,8-tetrahydrofolate + L-Ser | - |
Ovis aries | 5,10-methylenetetrahydrofolate + glycine + H2O | - |
r |
Subunits | Comment | Organism |
---|---|---|
tetramer | 4 * 53000, SDS-PAGE | Ovis aries |
Synonyms | Comment | Organism |
---|---|---|
SHMT | - |
Ovis aries |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
55 | - |
Tm-value for wild-type enzyme and mutant enzymes R262A, S52C and S52A without ligands or with Gly as ligand. Tm-value for mutant enzymes mutant enzymes R262A, S52C and S52A with L-Ser as ligand | Ovis aries |
68 | - |
Tm-value for wild-type enzyme with L-Ser as ligand | Ovis aries |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.2 | - |
L-Ser | pH 7.4, 37°C, mutant enzyme S52C | Ovis aries | |
0.7 | - |
L-Ser | pH 7.4, 37°C, mutant enzyme S52A | Ovis aries | |
0.8 | - |
L-Ser | pH 7.4, 37°C, mutant enzyme R262A | Ovis aries | |
4.2 | - |
L-Ser | pH 7.4, 37°C, wild-type enzyme | Ovis aries |