Protein Variants | Comment | Organism |
---|---|---|
C203S | no loss of activity | Ovis aries |
D227N | nearly complete loss of activity, enzyme exists as dimer | Ovis aries |
H304A | nearly complete loss of activity, enzyme exists as dimer | Ovis aries |
H306A | partial loss of activity, 60% of the enzyme exists as dimer | Ovis aries |
H356A | partial loss of activity, 80% of the enzyme exists as dimer | Ovis aries |
K71Q | nearly complete loss of activity, enzyme exists as dimer | Ovis aries |
R80A | nearly complete loss of activity, enzyme exists as dimer | Ovis aries |
R98A | mutant enzyme is present in the insoluble fraction | Ovis aries |
S202C | partial loss of activity, 55% of the enzyme exists as dimer | Ovis aries |
W110A | mutant enzyme is present in the insoluble fraction | Ovis aries |
W110F | no loss of activity | Ovis aries |
Y72F | partial loss of activity, 30% of the enzyme exists as dimer | Ovis aries |
Y81F | 20% of the enzyme exists as dimer | Ovis aries |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
cytosol | - |
Ovis aries | 5829 | - |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
53000 | - |
4 * 53000, wild-type enzyme and mutant enzymes C203F and W110F, SDS-PAGE | Ovis aries |
210000 | - |
gel filtration | Ovis aries |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Ovis aries | - |
- |
- |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
liver | - |
Ovis aries | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
L-Ser + tetrahydrofolate | - |
Ovis aries | Gly + 5,10-methylenetetrahydrofolate | - |
? |
Subunits | Comment | Organism |
---|---|---|
tetramer | 4 * 53000, wild-type enzyme and mutant enzymes C203F and W110F, SDS-PAGE | Ovis aries |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
pyridoxal 5'-phosphate | dimeric form of some mutant enzymes contains no pyridoxal 5'-phosphate | Ovis aries |