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Literature summary for 2.1.2.1 extracted from

  • Jala, V.R.; Appaji Rao, N.; Savithri, H.S.
    Identification of amino acid residues, essential for maintaining the tetrameric structure of sheep liver cytosolic serine hydroxymethyltransferase, by targeted mutagenesis (2003), Biochem. J., 369, 469-476.
    View publication on PubMedView publication on EuropePMC

Protein Variants

Protein Variants Comment Organism
C203S no loss of activity Ovis aries
D227N nearly complete loss of activity, enzyme exists as dimer Ovis aries
H304A nearly complete loss of activity, enzyme exists as dimer Ovis aries
H306A partial loss of activity, 60% of the enzyme exists as dimer Ovis aries
H356A partial loss of activity, 80% of the enzyme exists as dimer Ovis aries
K71Q nearly complete loss of activity, enzyme exists as dimer Ovis aries
R80A nearly complete loss of activity, enzyme exists as dimer Ovis aries
R98A mutant enzyme is present in the insoluble fraction Ovis aries
S202C partial loss of activity, 55% of the enzyme exists as dimer Ovis aries
W110A mutant enzyme is present in the insoluble fraction Ovis aries
W110F no loss of activity Ovis aries
Y72F partial loss of activity, 30% of the enzyme exists as dimer Ovis aries
Y81F 20% of the enzyme exists as dimer Ovis aries

Localization

Localization Comment Organism GeneOntology No. Textmining
cytosol
-
Ovis aries 5829
-

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
53000
-
4 * 53000, wild-type enzyme and mutant enzymes C203F and W110F, SDS-PAGE Ovis aries
210000
-
gel filtration Ovis aries

Organism

Organism UniProt Comment Textmining
Ovis aries
-
-
-

Source Tissue

Source Tissue Comment Organism Textmining
liver
-
Ovis aries
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
L-Ser + tetrahydrofolate
-
Ovis aries Gly + 5,10-methylenetetrahydrofolate
-
?

Subunits

Subunits Comment Organism
tetramer 4 * 53000, wild-type enzyme and mutant enzymes C203F and W110F, SDS-PAGE Ovis aries

Cofactor

Cofactor Comment Organism Structure
pyridoxal 5'-phosphate dimeric form of some mutant enzymes contains no pyridoxal 5'-phosphate Ovis aries