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Literature summary for 2.1.2.1 extracted from
- cDNA cloning, overexpression in Escherichia coli, purification and characterization of sheep liver cytosolic serine hydroxymethyltransferase (1995), Eur. J. Biochem., 230, 533-537.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| expression in Escherichia coli | Ovis aries |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| D-alanine | - |
Ovis aries |  |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.82 | - |
tetrahydrofolate | recombinant enzyme | Ovis aries | |
| 1 | - |
L-serine | recombinant enzyme | Ovis aries | |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| cytoplasm | - |
Ovis aries | 5737 | - |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 53000 | - |
4 * 53000, SDS-PAGE | Ovis aries |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| L-serine + tetrahydrofolate | Ovis aries | enzyme is a component of thymidylate cycle | glycine + 5,10-methylenetetrahydrofolate + H2O | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Ovis aries | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| recombinant enzyme | Ovis aries |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| additional information | - |
- |
Ovis aries |
| 0.02 | - |
crude extract | Ovis aries |
| 0.6 | - |
recombinant enzyme, crude extract | Ovis aries |
| 3.2 | - |
purified recombinant enzyme | Ovis aries |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| L-serine + tetrahydrofolate | - |
Ovis aries | glycine + 5,10-methylenetetrahydrofolate + H2O | - |
r | |
| L-serine + tetrahydrofolate | enzyme is a component of thymidylate cycle | Ovis aries | glycine + 5,10-methylenetetrahydrofolate + H2O | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| homotetramer | 4 * 53000, SDS-PAGE | Ovis aries |
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