General Stability | Organism |
---|---|
DTT stabilizes | Saccharomyces cerevisiae |
pyridoxal 5'-phosphate stabilizes | Saccharomyces cerevisiae |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
additional information | not: EDTA; not: purine nucleoside mono-, di- and triphosphates | Saccharomyces cerevisiae |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
additional information | - |
additional information | - |
Saccharomyces cerevisiae | |
0.15 | 0.9 | L-serine | - |
Saccharomyces cerevisiae |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Ca2+ | activation at 0.5-10 mM | Saccharomyces cerevisiae | |
Mg2+ | activation at 0.5-10 mM | Saccharomyces cerevisiae | |
Mn2+ | activation at 0.5-10 mM | Saccharomyces cerevisiae |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
180000 | 200000 | gel filtration, sucrose density gradient centrifugation | Saccharomyces cerevisiae |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
L-serine + tetrahydrofolate | Saccharomyces cerevisiae | key enzyme of serine pathway for assimilation of C1-compounds | glycine + 5,10-methylenetetrahydrofolate + H2O | - |
? | |
L-serine + tetrahydrofolate | Saccharomyces cerevisiae 3701B | key enzyme of serine pathway for assimilation of C1-compounds | glycine + 5,10-methylenetetrahydrofolate + H2O | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Saccharomyces cerevisiae | - |
- |
- |
Saccharomyces cerevisiae 3701B | - |
- |
- |
Purification (Comment) | Organism |
---|---|
- |
Saccharomyces cerevisiae |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
additional information | - |
- |
Saccharomyces cerevisiae |
Storage Stability | Organism |
---|---|
-10°C, 50% loss of activity in 10 h | Saccharomyces cerevisiae |
4°C, 50% loss of activity in 10 h | Saccharomyces cerevisiae |
frozen, up to a month in the presence of 30% v/v glycerol | Saccharomyces cerevisiae |
glycerol, 30% v/v, enhances thermal stability | Saccharomyces cerevisiae |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
L-serine + tetrahydrofolate | - |
Saccharomyces cerevisiae | glycine + 5,10-methylenetetrahydrofolate + H2O | - |
? | |
L-serine + tetrahydrofolate | key enzyme of serine pathway for assimilation of C1-compounds | Saccharomyces cerevisiae | glycine + 5,10-methylenetetrahydrofolate + H2O | - |
? | |
L-serine + tetrahydrofolate | - |
Saccharomyces cerevisiae 3701B | glycine + 5,10-methylenetetrahydrofolate + H2O | - |
? | |
L-serine + tetrahydrofolate | key enzyme of serine pathway for assimilation of C1-compounds | Saccharomyces cerevisiae 3701B | glycine + 5,10-methylenetetrahydrofolate + H2O | - |
? |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
additional information | - |
glycerol, 30% v/v, enhances thermal stability | Saccharomyces cerevisiae |
55 | - |
t1/2: about 2 min, glycerol stabilizes | Saccharomyces cerevisiae |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
8 | 9.5 | - |
Saccharomyces cerevisiae |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
pyridoxal 5'-phosphate | - |
Saccharomyces cerevisiae | |
tetrahydrofolate | requirement with L-serine or L-2-methylserine as substrate | Saccharomyces cerevisiae |