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Literature summary for 2.1.2.1 extracted from
- Purification and characterization of a serine hydroxymethyltransferase from an obligate methylotroph, Hyphomicrobium methylovorum GM2 (1987), Eur. J. Biochem., 162, 533-540.
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| 1,10-phenanthroline | - |
Hyphomicrobium methylovorum |  |
| 2,2'-dipyridyl | - |
Hyphomicrobium methylovorum | |
| 5,5-dithiobis(2-nitrobenzoic acid) | - |
Hyphomicrobium methylovorum | |
| Ag+ | strong | Hyphomicrobium methylovorum | |
| Cu2+ | strong | Hyphomicrobium methylovorum | |
| D-cycloserine | - |
Hyphomicrobium methylovorum | |
| Hg2+ | strong | Hyphomicrobium methylovorum | |
| KCl | - |
Hyphomicrobium methylovorum | |
| KCN | - |
Hyphomicrobium methylovorum | |
| LiCl | - |
Hyphomicrobium methylovorum | |
| additional information | not: EDTA | Hyphomicrobium methylovorum | |
| NaCl | - |
Hyphomicrobium methylovorum | |
| NaF | - |
Hyphomicrobium methylovorum | |
| NH2OH | - |
Hyphomicrobium methylovorum | |
| Pb2+ | strong | Hyphomicrobium methylovorum | |
| PCMB | strong | Hyphomicrobium methylovorum | |
| Penicillamine | - |
Hyphomicrobium methylovorum | |
| phenylhydrazine | partially reversible by pyridoxal 5'-phosphate | Hyphomicrobium methylovorum | |
| Semicarbazide | - |
Hyphomicrobium methylovorum | |
| Sodium borohydride | - |
Hyphomicrobium methylovorum | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| additional information | - |
additional information | - |
Hyphomicrobium methylovorum | |
| 0.04 | 0.046 | tetrahydrofolate | glycine | Hyphomicrobium methylovorum | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 48000 | - |
2 * 48000, SDS-PAGE | Hyphomicrobium methylovorum |
| 80000 | - |
HPLC gel filtration | Hyphomicrobium methylovorum |
| 98000 | - |
gel filtration | Hyphomicrobium methylovorum |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| L-serine + tetrahydrofolate | Hyphomicrobium methylovorum | key enzyme of serine pathway for assimilation of C1-compounds | glycine + 5,10-methylenetetrahydrofolate + H2O | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Hyphomicrobium methylovorum | - |
strain GM2, a glycine resistant mutant derived from strain KM146 | - |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Hyphomicrobium methylovorum |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 7.9 | - |
serine synthesis | Hyphomicrobium methylovorum |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| L-serine + tetrahydrofolate | - |
Hyphomicrobium methylovorum | glycine + 5,10-methylenetetrahydrofolate + H2O | - |
r | |
| L-serine + tetrahydrofolate | key enzyme of serine pathway for assimilation of C1-compounds | Hyphomicrobium methylovorum | glycine + 5,10-methylenetetrahydrofolate + H2O | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| dimer | 2 * 48000, SDS-PAGE | Hyphomicrobium methylovorum |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 37 | - |
serine synthesis | Hyphomicrobium methylovorum |
| 37 | 40 | 3-phenylserine degradation | Hyphomicrobium methylovorum |
Temperature Range [°C]
| Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 29 | 42 | serine synthesis, about half-maximal activity at 28.5°C and 90% of maximal activity at 42°C | Hyphomicrobium methylovorum |
| 30 | 42 | 2-phenylserine degradation, about half-maximal activity at 29.5°C and 80% of maximal activity at 42°C | Hyphomicrobium methylovorum |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 40 | - |
below, at least 10 min stable | Hyphomicrobium methylovorum |
| 45 | - |
above, rapid loss of activity | Hyphomicrobium methylovorum |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| additional information | - |
- |
Hyphomicrobium methylovorum |
| 7.5 | 8 | 3-phenylserine degradation | Hyphomicrobium methylovorum |
| 8 | - |
serine synthesis | Hyphomicrobium methylovorum |
pH Range
| pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|
| 7 | 9 | serine synthesis: about 80% of maximal activity at pH 7.0 and 9.0 | Hyphomicrobium methylovorum |
| 7.2 | 8.6 | 3-phenylserine degradation: about half-maximal activity at pH 7.2 and 8.6 | Hyphomicrobium methylovorum |
pH Stability
| pH Stability | pH Stability Maximum | Comment | Organism |
|---|---|---|---|
| 6 | 7.5 | stable | Hyphomicrobium methylovorum |
Cofactor
| Cofactor | Comment | Organism | Structure |
|---|---|---|---|
| pyridoxal 5'-phosphate | 2 mol per mol enzyme | Hyphomicrobium methylovorum | |
| tetrahydrofolate | requirement with L-serine or L-2-methylserine as substrate | Hyphomicrobium methylovorum | |
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