Activating Compound | Comment | Organism | Structure |
---|---|---|---|
additional information | in the presence of 1mM dithiothreitol + 1mM pyridoxal 5-phosphate + 20 mM EDTA: 29fold increase in the specific activity | Macaca radiata |
General Stability | Organism |
---|---|
2-mercaptoethanol stabilizes | Macaca radiata |
DTT stabilizes | Macaca radiata |
EDTA stabilizes | Macaca radiata |
pyridoxal 5'-phosphate stabilizes | Macaca radiata |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
aminopterin | - |
Macaca radiata | |
D-cycloserine | - |
Macaca radiata | |
Dichloromethotrexate | - |
Macaca radiata | |
dihydrofolate | - |
Macaca radiata | |
glycine | - |
Macaca radiata | |
methotrexate | - |
Macaca radiata | |
additional information | inhibition kinetics | Macaca radiata | |
tetrahydrofolate derivatives | - |
Macaca radiata |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
additional information | - |
additional information | allosteric kinetics | Macaca radiata |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
cytoplasm | 75% of the enzyme activity | Macaca radiata | 5737 | - |
microsome | 3% of the enzyme activity | Macaca radiata | - |
- |
mitochondrion | 5% of the enzyme activity | Macaca radiata | 5739 | - |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
52000 | - |
4 * 52000, SDS-PAGE | Macaca radiata |
208000 | - |
gel filtration | Macaca radiata |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
L-serine + tetrahydrofolate | Macaca radiata | regulatory protein | glycine + 5,10-methylenetetrahydrofolate + H2O | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Macaca radiata | - |
Bonnet monkey | - |
Purification (Comment) | Organism |
---|---|
heat-denaturation omitted | Macaca radiata |
Reaction | Comment | Organism | Reaction ID |
---|---|---|---|
5,10-methylenetetrahydrofolate + glycine + H2O = tetrahydrofolate + L-serine | mechanism | Macaca radiata |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
brain | - |
Macaca radiata | - |
kidney | - |
Macaca radiata | - |
additional information | distribution | Macaca radiata | - |
pancreas | - |
Macaca radiata | - |
skeletal muscle | - |
Macaca radiata | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
additional information | - |
- |
Macaca radiata |
3.4 | - |
- |
Macaca radiata |
Storage Stability | Organism |
---|---|
-20°C, 6-8 months in the presence of pyridoxal 5'-phosphate, 2-mercaptoethanol, DTT and EDTA | Macaca radiata |
0°C, at least 2 months in the presence of pyridoxal 5'-phosphate, 2-mercaptoethanol, DTT and EDTA | Macaca radiata |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
L-serine + tetrahydrofolate | - |
Macaca radiata | glycine + 5,10-methylenetetrahydrofolate + H2O | - |
? | |
L-serine + tetrahydrofolate | regulatory protein | Macaca radiata | glycine + 5,10-methylenetetrahydrofolate + H2O | - |
? | |
additional information | insoluble enzyme/antibody-complex shows 90% of original activity | Macaca radiata | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
homotetramer | 4 * 52000, SDS-PAGE | Macaca radiata |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
60 | - |
- |
Macaca radiata |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
additional information | - |
L-serine protects against thermal inactivation | Macaca radiata |
additional information | - |
enzyme-antibody complex more stable to elevated temperatures than free enzyme, allosteric effectors fail to protect free enzyme | Macaca radiata |
50 | - |
20 min stable | Macaca radiata |
65 | - |
85% loss of activity after 20 min | Macaca radiata |
70 | - |
complete loss of activity after 6 min, enzyme-antibody complex: 30% loss of activity after 20 min | Macaca radiata |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
additional information | - |
additional information | - |
Macaca radiata |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
additional information | - |
pI: 4.2 | Macaca radiata |
7.4 | - |
- |
Macaca radiata |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
pyridoxal 5'-phosphate | requirement, 4 mol per mol enzyme | Macaca radiata | |
tetrahydrofolate | - |
Macaca radiata |