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Literature summary for 2.1.2.1 extracted from
- Purification and characterization of cytosolic and mitochondrial serine hydroxymethyltransferases from rat liver (1981), J. Biochem., 90, 381-390.
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| D-alanine | inactivates enzyme by converting the enzyme bound pyridoxal 5'-phosphate to pyridoxamine phosphate in a transamination reaction | Rattus norvegicus |  |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| cytoplasm | - |
Rattus norvegicus | 5737 | - |
| mitochondrion | - |
Rattus norvegicus | 5739 | - |
| additional information | isozymes in cytosol and mitochondria | Rattus norvegicus | - |
- |
| additional information | antigenically related | Rattus norvegicus | - |
- |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 53700 | - |
4 * 53700, mitochondria, SDS-PAGE | Rattus norvegicus |
| 56300 | - |
4 * 56300, cytosol, SDS-PAGE | Rattus norvegicus |
| 200000 | 204000 | mitochondria, gel filtration, PAGE, amino acid composition | Rattus norvegicus |
| 216000 | - |
cytosol, PAGE | Rattus norvegicus |
| 230000 | - |
cytosol, amino acid composition, gel filtration | Rattus norvegicus |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| L-serine + tetrahydrofolate | Rattus norvegicus | catalyzes interconversion of serine and glycine | glycine + 5,10-methylenetetrahydrofolate + H2O | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Rattus norvegicus | - |
Wistar strain | - |
| Rattus norvegicus Wistar | - |
Wistar strain | - |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Rattus norvegicus |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 7.19 | - |
- |
Rattus norvegicus |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| L-serine + tetrahydrofolate | - |
Rattus norvegicus | glycine + 5,10-methylenetetrahydrofolate + H2O | - |
r | |
| L-serine + tetrahydrofolate | catalyzes interconversion of serine and glycine | Rattus norvegicus | glycine + 5,10-methylenetetrahydrofolate + H2O | - |
? | |
| additional information | no: L-threonine | Rattus norvegicus | ? | - |
? | |
| additional information | no: D-serine | Rattus norvegicus | ? | - |
? | |
| additional information | enzyme catalyzes the pyridoxal 5'-phosphate dependent reversible cleavage of 3-hydroxy-alpha-amino acids | Rattus norvegicus | ? | - |
? | |
| additional information | enzyme transaminates D-alanine to pyruvate and pyridoxamine phosphate | Rattus norvegicus | ? | - |
? | |
| additional information | no: D-allothreonine | Rattus norvegicus | ? | - |
? | |
| additional information | no: L-threonine | Rattus norvegicus Wistar | ? | - |
? | |
| additional information | no: D-serine | Rattus norvegicus Wistar | ? | - |
? | |
| additional information | enzyme catalyzes the pyridoxal 5'-phosphate dependent reversible cleavage of 3-hydroxy-alpha-amino acids | Rattus norvegicus Wistar | ? | - |
? | |
| additional information | enzyme transaminates D-alanine to pyruvate and pyridoxamine phosphate | Rattus norvegicus Wistar | ? | - |
? | |
| additional information | no: D-allothreonine | Rattus norvegicus Wistar | ? | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| homotetramer | 4 * 56300, cytosol, SDS-PAGE | Rattus norvegicus |
| homotetramer | 4 * 53700, mitochondria, SDS-PAGE | Rattus norvegicus |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| additional information | - |
pI, cytosolic enzyme: 4.95, pI, mitochondrial enzyme: 5.3 | Rattus norvegicus |
Cofactor
| Cofactor | Comment | Organism | Structure |
|---|---|---|---|
| pyridoxal 5'-phosphate | requirement, 4 mol per mol enzyme | Rattus norvegicus | |
| tetrahydrofolate | - |
Rattus norvegicus | |
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