Cloned (Comment) | Organism |
---|---|
expression in Escherichia coli | Carapichea ipecacuanha |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
Co2+ | 5 mM, 53% inhibition | Carapichea ipecacuanha | |
Mn2+ | 5 mM, 31% inhibition | Carapichea ipecacuanha | |
additional information | EDTA has no effect on activity, negligibly inhibited by iodoacetamide | Carapichea ipecacuanha | |
Ni2+ | 5 mM, 83% inhibition | Carapichea ipecacuanha | |
Zn2+ | 5 mM, 90% inhibition | Carapichea ipecacuanha |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.068 | - |
cephaeline | pH 6.8, 30°C | Carapichea ipecacuanha | |
0.097 | - |
S-adenosyl-L-methionine | pH 6.8, 30°C | Carapichea ipecacuanha |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
additional information | CiOMT does not require divalent cations for activity. Ca2+ and Mg2+ have no effect on activity | Carapichea ipecacuanha |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
40000 | - |
x * 40000, SDS-PAGE | Carapichea ipecacuanha |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
2 S-adenosyl-L-methionine + 7'-O-demethylcephaeline | Carapichea ipecacuanha | the enzyme is involved in biosynthesis of emetine | 2 S-adenosyl-L-homocysteine + emetine | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Carapichea ipecacuanha | E7FL15 | - |
- |
Purification (Comment) | Organism |
---|---|
- |
Carapichea ipecacuanha |
Reaction | Comment | Organism | Reaction ID |
---|---|---|---|
2 S-adenosyl-L-methionine + 7'-O-demethylcephaeline = 2 S-adenosyl-L-homocysteine + emetine | overall reaction | Carapichea ipecacuanha | |
S-adenosyl-L-methionine + 7'-O-demethylcephaeline = S-adenosyl-L-homocysteine + cephaeline | (1a) | Carapichea ipecacuanha | |
S-adenosyl-L-methionine + cephaeline = S-adenosyl-L-homocysteine + emetine | (1b) | Carapichea ipecacuanha |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
adventitious root culture | - |
Carapichea ipecacuanha | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
2 S-adenosyl-L-methionine + 7'-O-demethylcephaeline | the enzyme is involved in biosynthesis of emetine | Carapichea ipecacuanha | 2 S-adenosyl-L-homocysteine + emetine | - |
? | |
2 S-adenosyl-L-methionine + 7'-O-demethylcephaeline | the enzyme is involved in biosynthesis of emetine. Almost all of the 7'-O-demethylcephaeline is converted to cephaline within 2 min. Emetine the methylation product of cephaline is not observed, which indicates thatCiOMT1 does not use cephaline as a preferred substrate compared to 7'-O-demethylcephaeline. When all of the 7'-O-demethylcephaeline is converted to cephaline after 10 min, emetine is gradually produced from cephaline | Carapichea ipecacuanha | 2 S-adenosyl-L-homocysteine + emetine | - |
? | |
S-adenosyl-L-methionine + 7'-O-demethylcephaeline | - |
Carapichea ipecacuanha | S-adenosyl-L-homocysteine + cephaeline | - |
? | |
S-adenosyl-L-methionine + cephaeline | - |
Carapichea ipecacuanha | 2 S-adenosyl-L-homocysteine + emetine | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 40000, SDS-PAGE | Carapichea ipecacuanha |
Synonyms | Comment | Organism |
---|---|---|
7'-O-demethylcephaeline O-dimethyltransferase | - |
Carapichea ipecacuanha |
7'-O-demethylcephaeline/cephaeline 6',7'-methyltransferase | - |
Carapichea ipecacuanha |
Ci6'/7'OMT | - |
Carapichea ipecacuanha |
CiOMT1 | - |
Carapichea ipecacuanha |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
30 | - |
assay at | Carapichea ipecacuanha |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
6.8 | - |
cephaline as substrate | Carapichea ipecacuanha |