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Literature summary for 2.1.1.B83 extracted from

  • Cheong, B.E.; Takemura, T.; Yoshimatsu, K.; Sato, F.
    Molecular cloning of an O-methyltransferase from adventitious roots of Carapichea ipecacuanha (2011), Biosci. Biotechnol. Biochem., 75, 107-113.
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
expression in Escherichia coli Carapichea ipecacuanha

Inhibitors

Inhibitors Comment Organism Structure
Co2+ 5 mM, 53% inhibition Carapichea ipecacuanha
Mn2+ 5 mM, 31% inhibition Carapichea ipecacuanha
additional information EDTA has no effect on activity, negligibly inhibited by iodoacetamide Carapichea ipecacuanha
Ni2+ 5 mM, 83% inhibition Carapichea ipecacuanha
Zn2+ 5 mM, 90% inhibition Carapichea ipecacuanha

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.068
-
cephaeline pH 6.8, 30°C Carapichea ipecacuanha
0.097
-
S-adenosyl-L-methionine pH 6.8, 30°C Carapichea ipecacuanha

Metals/Ions

Metals/Ions Comment Organism Structure
additional information CiOMT does not require divalent cations for activity. Ca2+ and Mg2+ have no effect on activity Carapichea ipecacuanha

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
40000
-
x * 40000, SDS-PAGE Carapichea ipecacuanha

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2 S-adenosyl-L-methionine + 7'-O-demethylcephaeline Carapichea ipecacuanha the enzyme is involved in biosynthesis of emetine 2 S-adenosyl-L-homocysteine + emetine
-
?

Organism

Organism UniProt Comment Textmining
Carapichea ipecacuanha E7FL15
-
-

Purification (Commentary)

Purification (Comment) Organism
-
Carapichea ipecacuanha

Reaction

Reaction Comment Organism Reaction ID
2 S-adenosyl-L-methionine + 7'-O-demethylcephaeline = 2 S-adenosyl-L-homocysteine + emetine overall reaction Carapichea ipecacuanha
S-adenosyl-L-methionine + 7'-O-demethylcephaeline = S-adenosyl-L-homocysteine + cephaeline (1a) Carapichea ipecacuanha
S-adenosyl-L-methionine + cephaeline = S-adenosyl-L-homocysteine + emetine (1b) Carapichea ipecacuanha

Source Tissue

Source Tissue Comment Organism Textmining
adventitious root culture
-
Carapichea ipecacuanha
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2 S-adenosyl-L-methionine + 7'-O-demethylcephaeline the enzyme is involved in biosynthesis of emetine Carapichea ipecacuanha 2 S-adenosyl-L-homocysteine + emetine
-
?
2 S-adenosyl-L-methionine + 7'-O-demethylcephaeline the enzyme is involved in biosynthesis of emetine. Almost all of the 7'-O-demethylcephaeline is converted to cephaline within 2 min. Emetine the methylation product of cephaline is not observed, which indicates thatCiOMT1 does not use cephaline as a preferred substrate compared to 7'-O-demethylcephaeline. When all of the 7'-O-demethylcephaeline is converted to cephaline after 10 min, emetine is gradually produced from cephaline Carapichea ipecacuanha 2 S-adenosyl-L-homocysteine + emetine
-
?
S-adenosyl-L-methionine + 7'-O-demethylcephaeline
-
Carapichea ipecacuanha S-adenosyl-L-homocysteine + cephaeline
-
?
S-adenosyl-L-methionine + cephaeline
-
Carapichea ipecacuanha 2 S-adenosyl-L-homocysteine + emetine
-
?

Subunits

Subunits Comment Organism
? x * 40000, SDS-PAGE Carapichea ipecacuanha

Synonyms

Synonyms Comment Organism
7'-O-demethylcephaeline O-dimethyltransferase
-
Carapichea ipecacuanha
7'-O-demethylcephaeline/cephaeline 6',7'-methyltransferase
-
Carapichea ipecacuanha
Ci6'/7'OMT
-
Carapichea ipecacuanha
CiOMT1
-
Carapichea ipecacuanha

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
30
-
assay at Carapichea ipecacuanha

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
6.8
-
cephaline as substrate Carapichea ipecacuanha