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Literature summary for 2.1.1.B76 extracted from

  • Kim, B.G.; Kim, H.; Hur, H.G.; Lim, Y.; Ahn, J.H.
    Regioselectivity of 7-O-methyltransferase of poplar to flavones (2006), J. Biotechnol., 126, 241-247.
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
expression in Escherichia coli as a glutathione S-transferase fusion protein Populus deltoides

Organism

Organism UniProt Comment Textmining
Populus deltoides
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Populus deltoides Marsh
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Reaction

Reaction Comment Organism Reaction ID
S-adenosyl-L-methionine + a flavone = S-adenosyl-L-homocysteine + a 7-methoxyflavone (1) Populus deltoides
S-adenosyl-L-methionine + a flavonol = S-adenosyl-L-homocysteine + a 7-methoxyflavonol (2) Populus deltoides

Source Tissue

Source Tissue Comment Organism Textmining
leaf
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Populus deltoides
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Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
additional information POMT-7 is able to transfer a methyl group to several flavonoids containing a C-7 hydroxyl group. POMT-7 shows a higher affinity toward flavonol and flavone such as apigenin, kaempferol, luteolin, and quercetin than flavanone and isoflavone Populus deltoides ?
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?
additional information POMT-7 is able to transfer a methyl group to several flavonoids containing a C-7 hydroxyl group. POMT-7 shows a higher affinity toward flavonol and flavone such as apigenin, kaempferol, luteolin, and quercetin than flavanone and isoflavone Populus deltoides Marsh ?
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?
S-adenosyl-L-methionine + (2S)-5,7,4'-trihydroxyflavanone i.e. (2S)-naringenin, 16% of the activity with apigenin (i.e. 5,7,4'-trihydroxyflavone) Populus deltoides S-adenosyl-L-homocysteine + (2S)-5,4'-dihydroxy-7-methoxyflavanone i.e. (2S)-sakuranetin ?
S-adenosyl-L-methionine + (2S)-5,7,4'-trihydroxyflavanone i.e. (2S)-naringenin, 16% of the activity with apigenin (i.e. 5,7,4'-trihydroxyflavone) Populus deltoides Marsh S-adenosyl-L-homocysteine + (2S)-5,4'-dihydroxy-7-methoxyflavanone i.e. (2S)-sakuranetin ?
S-adenosyl-L-methionine + 3',4',5,7-tetrahydroxyflavanone i.e. eriodictyol, 14% of the activity with apigenin (i.e. 5,7,4'-trihydroxyflavone) Populus deltoides S-adenosyl-L-homocysteine + 3,4',5-trihydroxy-7-methoxyflavanone
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?
S-adenosyl-L-methionine + 3,3',4',5,7-pentahydroxyflavone i.e. quercetin, 98% of the activity with apigenin (i.e. 5,7,4'-trihydroxyflavone) Populus deltoides S-adenosyl-L-homocysteine + 3,3',4',5-tetrahydroxy-7-methoxyflavone
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?
S-adenosyl-L-methionine + 3,3',4',5,7-pentahydroxyflavone i.e. quercetin, 98% of the activity with apigenin (i.e. 5,7,4'-trihydroxyflavone) Populus deltoides Marsh S-adenosyl-L-homocysteine + 3,3',4',5-tetrahydroxy-7-methoxyflavone
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?
S-adenosyl-L-methionine + 3,4',5,7-tetrahydroxyflavanone i.e. kaempferol, 82% of the activity with apigenin (i.e. 5,7,4'-trihydroxyflavone) Populus deltoides S-adenosyl-L-homocysteine + 3,4',5-trihydroxy-7-methoxyflavanone
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?
S-adenosyl-L-methionine + 3,5,7,4'-tetrahydroxy-3'-methoxyflavone i.e. isorhamnetin, 57% of the activity with apigenin (i.e. 5,7,4'-trihydroxyflavone) Populus deltoides S-adenosyl-L-homocysteine + 3,5,4'-trihydroxy-7,3'-dimethoxyflavone
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?
S-adenosyl-L-methionine + 3,5,7,4'-tetrahydroxy-3'-methoxyflavone i.e. isorhamnetin, 57% of the activity with apigenin (i.e. 5,7,4'-trihydroxyflavone) Populus deltoides Marsh S-adenosyl-L-homocysteine + 3,5,4'-trihydroxy-7,3'-dimethoxyflavone
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?
S-adenosyl-L-methionine + 5,7,4'-trihydroxyflavone i.e. apigenin Populus deltoides S-adenosyl-L-homocysteine + 5,4'-dihydroxy-7-methoxyflavone i.e. genkwanin ?
S-adenosyl-L-methionine + 5,7,4'-trihydroxyflavone i.e. apigenin Populus deltoides Marsh S-adenosyl-L-homocysteine + 5,4'-dihydroxy-7-methoxyflavone i.e. genkwanin ?
S-adenosyl-L-methionine + luteolin i.e. 5,7,3',4'-tetrahydroxyflavanone, 87% of the activity with apigenin (i.e. 5,7,4'-trihydroxyflavone) Populus deltoides S-adenosyl-L-homocysteine + 3,4',5-trihydroxy-7-methoxyflavanone
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?

Synonyms

Synonyms Comment Organism
flavonoid 7-O-methyltransferase
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Populus deltoides
POMT-7
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Populus deltoides