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Literature summary for 2.1.1.B3 extracted from

  • Kelm, A.; Shaw, L.; Schauer, R.; Reuter, G.
    The biosynthesis of 8-O-methylated sialic acids in the starfish Asterias rubens. Isolation and characterisation of S-adenosyl-L-methionine: sialate-8-O-methyltransferase (1998), Eur. J. Biochem., 251, 874-884.
    View publication on PubMed
Search for more literature for 2.1.1.B3

Inhibitors

Inhibitors Comment Organism Structure
Cu2+ complete inhibition at 2 mM Asterias rubens
EDTA complete inhibition at 1 mM, reversible by Mn2+ or, to a lesser extent, by Co2+ to 90% and 68% of maximal activity, respectively, no protection by Mg2+ Asterias rubens
Fe3+ 18% inhibition at 2 mM Asterias rubens
Ni2+ 84% inhibition at 2 mM Asterias rubens
Zn2+ 83% inhibition at 2 mM Asterias rubens

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.0071
-
 S-adenosyl-L-methionine pH 8.0, 25°C, with N-acetylneuraminyl alpha2,3-lactose Asterias rubens
0.044
-
 N-acetylneuraminyl alpha2,3-lactose pH 8.0, 25°C Asterias rubens
0.299
-
 N-acetylneuraminic acid pH 8.0, 25°C Asterias rubens

Localization

Localization Comment Organism GeneOntology No. Textmining
membrane
-
 Asterias rubens 16020
-

Metals/Ions

Metals/Ions Comment Organism Structure
Ca2+ activates at 2 mM Asterias rubens
Co2+ activates at 2 mM Asterias rubens
Mg2+ activates at 2 mM Asterias rubens
Mn2+ activates at 2 mM Asterias rubens

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
58000
-
 x * 58000 + x * 62000, SDS-PAGE Asterias rubens
62000
-
 x * 58000 + x * 62000, SDS-PAGE Asterias rubens

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
S-adenosyl-L-methionine + N-acetylneuraminic acid Asterias rubens
-
 S-adenosyl-L-homocysteine + N-acetyl-8-O-methyl-neuraminate
-
 ?

Organism

Organism UniProt Comment Textmining
Asterias rubens
-
-
-

Purification (Commentary)

Purification (Comment) Organism
native enzyme 22000fold from gonads to homogeneity by detergent-dependent solubilization, ion exchange chromatography, and two cycles of affinity chromatography on an S-adenosyl-L-homocysteine resin Asterias rubens

Source Tissue

Source Tissue Comment Organism Textmining
gonad
-
 Asterias rubens
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
71.6
-
 purified enzyme Asterias rubens

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
additional information the enzyme preforms specific methylation of endogenous and exogenous glycoconjugate-bound sialic acids, usage of desialized human erythrocyte membranes as substrates, methylation of resialylated erythrocyte membranes, overview Asterias rubens ?
-
 ?
S-adenosyl-L-methionine + CMP-N-acetylneuraminic acid
-
 Asterias rubens S-adenosyl-L-homocysteine + CMP-N-acetyl-8-O-methyl-neuraminate
-
 ?
S-adenosyl-L-methionine + colominic acid
-
 Asterias rubens ?
-
 ?
S-adenosyl-L-methionine + fetuin
-
 Asterias rubens ?
-
 ?
S-adenosyl-L-methionine + N-acetylneuraminic acid
-
 Asterias rubens S-adenosyl-L-homocysteine + N-acetyl-8-O-methyl-neuraminate
-
 ?
S-adenosyl-L-methionine + N-acetylneuraminic acid free N-acetylneuraminic acid and oligosaccharide- or glycoprotein-bound substrate, the latter is preferred Asterias rubens S-adenosyl-L-homocysteine + N-acetyl-8-O-methyl-neuraminate NMR product identification ?
S-adenosyl-L-methionine + N-acetylneuraminyl alpha2,3-lactose free N-acetylneuraminyl alpha2,3-lactose and oligosaccharide- or glycoprotein-bound substrate, the latter is preferred Asterias rubens S-adenosyl-L-homocysteine + N-acetyl-8-O-methyl-neuraminyl alpha2,3-lactose
-
 ?
S-adenosyl-L-methionine + N-acetylneuraminyl alpha2,6-lactose free N-acetylneuraminyl alpha2,6-lactose acid and oligosaccharide- or glycoprotein-bound substrate, the latter is preferred Asterias rubens S-adenosyl-L-homocysteine + N-acetyl-8-O-methyl-neuraminyl alpha2,6-lactose
-
 ?
S-adenosyl-L-methionine + N-glycoloylneuraminic acid free N-glycoloylneuraminic acid and oligosaccharide- or glycoprotein-bound substrate, the latter is preferred Asterias rubens S-adenosyl-L-homocysteine + N-glycoloyl-8-O-methyl-neuraminate
-
 ?

Subunits

Subunits Comment Organism
? x * 58000 + x * 62000, SDS-PAGE Asterias rubens

Synonyms

Synonyms Comment Organism
S-adenosyl-L-methionine: sialate-8-O-methyltransferase
-
 Asterias rubens
sialate-8-O-methyltransferase
-
 Asterias rubens

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
37
-
-
 Asterias rubens

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
37
-
 the purified enzyme is quite unstable at 37°C Asterias rubens

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.4 8.3 broad optimum Asterias rubens