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Literature summary for 2.1.1.95 extracted from

  • Tavva, V.S.; Kim, Y.H.; Kagan, I.A.; Dinkins, R.D.; Kim, K.H.; Collins, G.B.
    Increased alpha-tocopherol content in soybean seed overexpressing the Perilla frutescens gamma-tocopherol methyltransferase gene (2007), Plant Cell Rep., 26, 61-70.
    View publication on PubMed

Application

Application Comment Organism
nutrition increase in the alpha-tocopherol content in the soybean seed, due to transgenically modulated enzyme activity, could have a potential to significantly increase the dietary intake of vitamin E Perilla frutescens

Cloned(Commentary)

Cloned (Comment) Organism
expressed in soybean using a seed specific promoter, transgenic seeds contain a drastically enhanced amount of alpha-tocopherol compared with native seeds Perilla frutescens
gene PfTMT, overexpression in transgenic Glycine max plant seeds cloned in the pCAMBIA1304 binary vector under a seed-specific promoter, vicilin and the poly A sequence from the Agrobacterium tumefaciens octopine synthase gene Perilla frutescens

Protein Variants

Protein Variants Comment Organism
additional information overexpression of the enzyme leads to increased alpha-tocopherol content in transgenic Glycine max plant seeds, 10.4fold increased alpha-tocopherol content and 14.9fold increased beta-tocopherol content in T2 seeds, tocopherol contents in the seeds from T0 and T1 transgenic soybean plants, overview Perilla frutescens

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
S-adenosyl-L-methionine + delta-tocopherol Perilla frutescens
-
S-adenosyl-L-homocysteine + beta-tocopherol
-
?
S-adenosyl-L-methionine + gamma-tocopherol Perilla frutescens
-
S-adenosyl-L-homocysteine + alpha-tocopherol
-
?
S-adenosyl-L-methionine + gamma-tocopherol Perilla frutescens biosynthesis of alpha-tocopherol S-adenosyl-L-homocysteine + alpha-tocopherol
-
?

Organism

Organism UniProt Comment Textmining
Perilla frutescens Q8VWX2 cv.Okdong
-
Perilla frutescens Q8VWX2 cv. Okdong, gene PfTMT
-

Source Tissue

Source Tissue Comment Organism Textmining

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
S-adenosyl-L-methionine + delta-tocopherol
-
Perilla frutescens S-adenosyl-L-homocysteine + beta-tocopherol
-
?
S-adenosyl-L-methionine + gamma-tocopherol
-
Perilla frutescens S-adenosyl-L-homocysteine + alpha-tocopherol
-
?
S-adenosyl-L-methionine + gamma-tocopherol biosynthesis of alpha-tocopherol Perilla frutescens S-adenosyl-L-homocysteine + alpha-tocopherol
-
?

Synonyms

Synonyms Comment Organism
gamma-TMT
-
Perilla frutescens
gamma-tocopherol methyltransferase
-
Perilla frutescens

Cofactor

Cofactor Comment Organism Structure
S-adenosyl-L-methionine
-
Perilla frutescens