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Literature summary for 2.1.1.88 extracted from

  • Jay, M.; De Luca, V.; Ibrahim, R.K.
    Purification, properties and kinetic mechanism of flavonol 8-O-methyltransferase from Lotus corniculatus L (1985), Eur. J. Biochem., 153, 321-325.
    View publication on PubMed
Search for more literature for 2.1.1.88

Inhibitors

Inhibitors Comment Organism Structure
8-Methoxykaempferol product inhibition, competitive with respect to 8-hydroxykaempferol Lotus corniculatus
EDTA in absence of Mg2+ Lotus corniculatus
S-adenosyl-L-homocysteine product inhibition, non-competitive with respect to S-adenosyl-L-methionine Lotus corniculatus

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.0013
-
 8-Hydroxykaempferol
-
 Lotus corniculatus
0.053
-
 S-adenosyl-L-methionine
-
 Lotus corniculatus

Metals/Ions

Metals/Ions Comment Organism Structure
Mg2+ required Lotus corniculatus

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
55000
-
 gel filtration Lotus corniculatus

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
S-adenosyl-L-methionine + 3,3',4',5,7,8-hexahydroxyflavone Lotus corniculatus
-
 S-adenosyl-L-homocysteine + 3,3',4',5,7-pentahydroxy-8-methoxyflavone
-
 ?
S-adenosyl-L-methionine + 8-hydroxykaempferol Lotus corniculatus
-
 S-adenosyl-L-homocysteine + 8-methoxykaempferol
-
 ?

Organism

Organism UniProt Comment Textmining
Lotus corniculatus
-
-
-

Purification (Commentary)

Purification (Comment) Organism
1230fold Lotus corniculatus

Reaction

Reaction Comment Organism Reaction ID
S-adenosyl-L-methionine + 3,5,7,8,3',4'-hexahydroxyflavone = S-adenosyl-L-homocysteine + 3,5,7,3',4'-pentahydroxy-8-methoxyflavone mechanism Lotus corniculatus
a

Source Tissue

Source Tissue Comment Organism Textmining
bud
-
 Lotus corniculatus
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
additional information
-
-
 Lotus corniculatus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
additional information not: glycosides of 8-hydroxyflavonols Lotus corniculatus ?
-
 ?
S-adenosyl-L-methionine + 3,3',4',5,7,8-hexahydroxyflavone
-
 Lotus corniculatus S-adenosyl-L-homocysteine + 3,3',4',5,7-pentahydroxy-8-methoxyflavone
-
 ?
S-adenosyl-L-methionine + 3,3',4',5,7,8-hexahydroxyflavone i.e. 8-hydroxyquercetin Lotus corniculatus S-adenosyl-L-homocysteine + 3,3',4',5,7-pentahydroxy-8-methoxyflavone i.e. 8-methoxyquercetin ?
S-adenosyl-L-methionine + 8-hydroxykaempferol
-
 Lotus corniculatus S-adenosyl-L-homocysteine + 8-methoxykaempferol
-
 ?

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.9
-
-
 Lotus corniculatus