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Literature summary for 2.1.1.86 extracted from
- Sodium ion translocation by N5-methyltetrahydromethanopterin: coenzyme M methyltransferase from Methanosarcina mazei Gö1 reconstituted in ether lipid liposomes (1996), Eur. J. Biochem., 239, 857-864.
General Stability
| General Stability | Organism |
|---|---|
| during purification, the enzyme is stabilized with 0.01 mM hydroxocobalamin | Methanosarcina mazei |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| membrane | membrane associated enzyme | Methanosarcina mazei | 16020 | - |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Na+ | maximum activity at 1 mM Na+ | Methanosarcina mazei |  |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 9000 | - |
x * 34000 + x * 28000 + x * 20000 + x * 13000 + x * 12000 + x * 9000, SDS-PAGE | Methanosarcina mazei |
| 12000 | - |
x * 34000 + x * 28000 + x * 20000 + x * 13000 + x * 12000 + x * 9000, SDS-PAGE | Methanosarcina mazei |
| 13000 | - |
x * 34000 + x * 28000 + x * 20000 + x * 13000 + x * 12000 + x * 9000, SDS-PAGE | Methanosarcina mazei |
| 20000 | - |
x * 34000 + x * 28000 + x * 20000 + x * 13000 + x * 12000 + x * 9000, SDS-PAGE | Methanosarcina mazei |
| 28000 | - |
x * 34000 + x * 28000 + x * 20000 + x * 13000 + x * 12000 + x * 9000, SDS-PAGE | Methanosarcina mazei |
| 34000 | - |
x * 34000 + x * 28000 + x * 20000 + x * 13000 + x * 12000 + x * 9000, SDS-PAGE | Methanosarcina mazei |
| 380000 | - |
non-denaturing gradient PAGE | Methanosarcina mazei |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 5-methyl-5,6,7,8-tetrahydromethanopterin + 2-mercaptoethanesulfonate | Methanosarcina mazei | 2-mercaptoethanesulfonate i.e. coenzyme M. The enzyme uses the methylation of coenzyme M (HS-CoM) by methyl-tetrahydromethanopterin to drive an energy-conserving sodium ion pump | 5,6,7,8-tetrahydromethanopterin + 2-(methylthio)ethanesulfonate | - |
? | |
| 5-methyl-5,6,7,8-tetrahydromethanopterin + 2-mercaptoethanesulfonate | Methanosarcina mazei DSM 3647 | 2-mercaptoethanesulfonate i.e. coenzyme M. The enzyme uses the methylation of coenzyme M (HS-CoM) by methyl-tetrahydromethanopterin to drive an energy-conserving sodium ion pump | 5,6,7,8-tetrahydromethanopterin + 2-(methylthio)ethanesulfonate | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Methanosarcina mazei | P80655 and P80653 and P80651 and P80654 and P80656 and P80650 | P80655: subunit B, P80653: subunit D, P80651: subunit E, P80654: subunit F, P80656: subunit G, P80650: subunit H | - |
| Methanosarcina mazei DSM 3647 | P80655 and P80653 and P80651 and P80654 and P80656 and P80650 | P80655: subunit B, P80653: subunit D, P80651: subunit E, P80654: subunit F, P80656: subunit G, P80650: subunit H | - |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Methanosarcina mazei |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| culture condition:acetate-grown cell | - |
Methanosarcina mazei | - |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 10.4 | - |
37°C, pH 6.9 | Methanosarcina mazei |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 5-methyl-5,6,7,8-tetrahydromethanopterin + 2-mercaptoethanesulfonate | 2-mercaptoethanesulfonate i.e. coenzyme M. The enzyme uses the methylation of coenzyme M (HS-CoM) by methyl-tetrahydromethanopterin to drive an energy-conserving sodium ion pump | Methanosarcina mazei | 5,6,7,8-tetrahydromethanopterin + 2-(methylthio)ethanesulfonate | - |
? | |
| 5-methyl-5,6,7,8-tetrahydromethanopterin + 2-mercaptoethanesulfonate | 2-mercaptoethanesulfonate i.e. coenzyme M. The enzyme uses the methylation of coenzyme M (HS-CoM) by methyl-tetrahydromethanopterin to drive an energy-conserving sodium ion pump. 1.7 mol Na+ per mol of methyl groups transferred are translocated | Methanosarcina mazei | 5,6,7,8-tetrahydromethanopterin + 2-(methylthio)ethanesulfonate | - |
? | |
| 5-methyl-5,6,7,8-tetrahydromethanopterin + 2-mercaptoethanesulfonate | 2-mercaptoethanesulfonate i.e. coenzyme M. The enzyme uses the methylation of coenzyme M (HS-CoM) by methyl-tetrahydromethanopterin to drive an energy-conserving sodium ion pump | Methanosarcina mazei DSM 3647 | 5,6,7,8-tetrahydromethanopterin + 2-(methylthio)ethanesulfonate | - |
? | |
| 5-methyl-5,6,7,8-tetrahydromethanopterin + 2-mercaptoethanesulfonate | 2-mercaptoethanesulfonate i.e. coenzyme M. The enzyme uses the methylation of coenzyme M (HS-CoM) by methyl-tetrahydromethanopterin to drive an energy-conserving sodium ion pump. 1.7 mol Na+ per mol of methyl groups transferred are translocated | Methanosarcina mazei DSM 3647 | 5,6,7,8-tetrahydromethanopterin + 2-(methylthio)ethanesulfonate | - |
? | |
| methylcobalamin + 2-mercaptoethanesulfonate | at 13.6% of the activity compared to 5-methyl-5,6,7,8-tetrahydromethanopterin | Methanosarcina mazei | ? | - |
? | |
| methylcobalamin + 2-mercaptoethanesulfonate | at 13.6% of the activity compared to 5-methyl-5,6,7,8-tetrahydromethanopterin | Methanosarcina mazei DSM 3647 | ? | - |
? | |
| N5-methyltetrahydrofolate + 2-mercaptoethanesulfonate | at 31.8% of the activity compared to 5-methyl-5,6,7,8-tetrahydromethanopterin | Methanosarcina mazei | ? | - |
? | |
| N5-methyltetrahydrofolate + 2-mercaptoethanesulfonate | at 31.8% of the activity compared to 5-methyl-5,6,7,8-tetrahydromethanopterin | Methanosarcina mazei DSM 3647 | ? | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| ? | x * 34000 + x * 28000 + x * 20000 + x * 13000 + x * 12000 + x * 9000, SDS-PAGE | Methanosarcina mazei |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| H,MPT:coenzyme M methyltransferase | - |
Methanosarcina mazei |
| N5-methyltetrahydromethanopterin: coenzyme M methyltransferase | - |
Methanosarcina mazei |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 37 | - |
assay at | Methanosarcina mazei |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7 | 7.5 | - |
Methanosarcina mazei |
Cofactor
| Cofactor | Comment | Organism | Structure |
|---|---|---|---|
| corrinoid | - |
Methanosarcina mazei | |
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