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Literature summary for 2.1.1.85 extracted from

  • Vijayasarathy, C.; Rao, B.S.N.
    Partial purification and characterisation of S-adenosylmethionine:protein-histidine N-methyltransferase from rabbit skeletal muscle (1987), Biochim. Biophys. Acta, 923, 156-165.
    View publication on PubMed
Search for more literature for 2.1.1.85

Activating Compound

Activating Compound Comment Organism Structure
additional information reducing agents e.g. mercaptoethanol, required for activity, 4fold stimulation Oryctolagus cuniculus
Triton X-100 stimulation at 1% Oryctolagus cuniculus

General Stability

General Stability Organism
2-mercaptoethanol or glycerol does not stabilize Oryctolagus cuniculus

Inhibitors

Inhibitors Comment Organism Structure
ATP 47% inhibition at 0.4 mM Oryctolagus cuniculus
Borate slight inhibition Oryctolagus cuniculus
Ca2+ 30% inhibition at 0.4 mM Oryctolagus cuniculus
EDTA slight inhibition Oryctolagus cuniculus
K+ 72% inhibition at 200 mM Oryctolagus cuniculus
Mg2+ 60% inhibition at 8 mM Oryctolagus cuniculus
N-ethylmaleimide 70% inhibition at 4 mM Oryctolagus cuniculus
Na+ 95% inhibition at 200 mM Oryctolagus cuniculus

Localization

Localization Comment Organism GeneOntology No. Textmining
myofibril
-
 Oryctolagus cuniculus 30016
-

Metals/Ions

Metals/Ions Comment Organism Structure
additional information no requirement for any metal ion Oryctolagus cuniculus

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
S-adenosyl-L-methionine + protein L-histidine Oryctolagus cuniculus
-
 S-adenosyl-L-homocysteine + protein Ntau-methyl-L-histidine
-
 ?

Organism

Organism UniProt Comment Textmining
Oryctolagus cuniculus
-
 rabbit
-

Purification (Commentary)

Purification (Comment) Organism
partial Oryctolagus cuniculus

Source Tissue

Source Tissue Comment Organism Textmining
skeletal muscle
-
 Oryctolagus cuniculus
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
additional information
-
 different fractions after ammonium sulfate precipitation Oryctolagus cuniculus

Storage Stability

Storage Stability Organism
-20°C Oryctolagus cuniculus
2°C, 4 days, 75% loss of activity, no stabilization by mercaptoethanol or glycerol Oryctolagus cuniculus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
S-adenosyl-L-methionine + protein L-histidine
-
 Oryctolagus cuniculus S-adenosyl-L-homocysteine + protein Ntau-methyl-L-histidine
-
 ?
S-adenosyl-L-methionine + protein L-histidine specifically methylates histidine residues of actin Oryctolagus cuniculus S-adenosyl-L-homocysteine + protein Ntau-methyl-L-histidine
-
 ?
S-adenosyl-L-methionine + protein L-histidine some activity with gluten Oryctolagus cuniculus S-adenosyl-L-homocysteine + protein Ntau-methyl-L-histidine
-
 ?
S-adenosyl-L-methionine + protein L-histidine negligible activity with: bovine serum albumin, fibrinogen, casein, histone type IIA, gelatin, human gamma-globulin Oryctolagus cuniculus S-adenosyl-L-homocysteine + protein Ntau-methyl-L-histidine
-
 ?

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
37
-
 assay at Oryctolagus cuniculus

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
40
-
 10 min, 75% loss of activity Oryctolagus cuniculus
50
-
 10 min, complete loss of activity Oryctolagus cuniculus

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8
-
-
 Oryctolagus cuniculus

pH Range

pH Minimum pH Maximum Comment Organism
6.8 9 about 50% of activity maximum at pH 6.8 and 9.0 Oryctolagus cuniculus