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Literature summary for 2.1.1.84 extracted from

  • De Luca, V.; Ibrahim, R.K.
    Enzymatic synthesis of polymethylated flavonols in Chrysosplenium americanum. I. Partial purification and some properties of S-adenosyl-L-methionine:flavonol 3-, 6-, 7-, and 4-O-methyltransferases (1985), Arch. Biochem. Biophys., 238, 596-605.
    View publication on PubMed
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Inhibitors

Inhibitors Comment Organism Structure
EDTA
-
 Chrysosplenium americanum
N-ethylmaleimide
-
 Chrysosplenium americanum
p-chloromercuribenzoate
-
 Chrysosplenium americanum

Metals/Ions

Metals/Ions Comment Organism Structure
Mg2+ activates Chrysosplenium americanum

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
57000
-
 gel filtration Chrysosplenium americanum

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
S-adenosyl-L-methionine + 3',4',5,6-tetrahydroxy-3,7-dimethoxyflavone Chrysosplenium americanum
-
 S-adenosyl-L-homocysteine + 3',4',5-trihydroxy-3,6,7-trimethoxyflavone
-
 ?

Organism

Organism UniProt Comment Textmining
Chrysosplenium americanum
-
-
-

Purification (Commentary)

Purification (Comment) Organism
ammonium sulfate, Sephacryl S-200, hydroxylapatite, chromatofocusing Chrysosplenium americanum

Source Tissue

Source Tissue Comment Organism Textmining
leaf
-
 Chrysosplenium americanum
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.64
-
-
 Chrysosplenium americanum

Storage Stability

Storage Stability Organism
-15°C, 0.2 M potassium phosphate, pH 8, 20% glycerol, 5 mM dithiothreitol, for several months, no loss of activity Chrysosplenium americanum
4°C, 4-6 weeks, 80% loss of activity Chrysosplenium americanum

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3,7,3'-trimethylquercetagetin + S-adenosyl-L-methionine
-
 Chrysosplenium americanum 3,6,7',3'-tetrametylquercetagetin + S-adenosyl-L-homocysteine
-
 ?
8-hydroxykaempferol + S-adenosyl-L-methionine 25% of activity with 3',4',5,6-tetrahydroxy-3,7-dimethoxyflavone Chrysosplenium americanum ?
-
 ?
S-adenosyl-L-methionine + 3',4',5,6-tetrahydroxy-3,7-dimethoxyflavone
-
 Chrysosplenium americanum S-adenosyl-L-homocysteine + 3',4',5-trihydroxy-3,6,7-trimethoxyflavone
-
 ?

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8.8 9
-
 Chrysosplenium americanum