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Literature summary for 2.1.1.82 extracted from

  • Joe, E.J.; Kim, B.G.; An, B.C.; Chong, Y.; Ahn, J.H.
    Engineering of flavonoid O-methyltransferase for a novel regioselectivity (2010), Mol. Cells, 30, 137-141.
    View publication on PubMed
Search for more literature for 2.1.1.82

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli BL21 cells Populus sp.

Protein Variants

Protein Variants Comment Organism
D257G the mutant methylates the 3-hydroxyl group of flavonols in addition to the 7-hydrdoxyl group Populus sp.
L101M the mutant shows wild type reactivity with kaempferol and quercetin Populus sp.

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.0298
-
 quercetin wild type enzyme, in 10 mM Tris/HCl buffer (pH 7.5), at 37°C Populus sp.
0.0324
-
 quercetin mutant enzyme D257G, in 10 mM Tris/HCl buffer (pH 7.5), at 37°C Populus sp.
0.0324
-
 kaempferol wild type enzyme, in 10 mM Tris/HCl buffer (pH 7.5), at 37°C Populus sp.
0.0378
-
 kaempferol mutant enzyme D257G, in 10 mM Tris/HCl buffer (pH 7.5), at 37°C Populus sp.

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
60000
-
 x * 60000, SDS-PAGE Populus sp.

Organism

Organism UniProt Comment Textmining
Populus sp.
-
-
-

Purification (Commentary)

Purification (Comment) Organism
GST affinity column chromatography Populus sp.

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
S-adenosyl-L-methionine + kaempferol
-
 Populus sp. 7-O-methylkaempferol
-
 ?
S-adenosyl-L-methionine + quercetin
-
 Populus sp. 7-O-methylquercetin
-
 ?

Subunits

Subunits Comment Organism
? x * 60000, SDS-PAGE Populus sp.

Synonyms

Synonyms Comment Organism
flavone 7-O-methyltransferase
-
 Populus sp.
MT7
-
 Populus sp.

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.064
-
 quercetin mutant enzyme D257G, in 10 mM Tris/HCl buffer (pH 7.5), at 37°C Populus sp.
0.064
-
 kaempferol wild type enzyme, in 10 mM Tris/HCl buffer (pH 7.5), at 37°C Populus sp.
0.07
-
 kaempferol mutant enzyme D257G, in 10 mM Tris/HCl buffer (pH 7.5), at 37°C Populus sp.
0.071
-
 quercetin wild type enzyme, in 10 mM Tris/HCl buffer (pH 7.5), at 37°C Populus sp.

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
17
-
 kaempferol mutant enzyme D257G, in 10 mM Tris/HCl buffer (pH 7.5), at 37°C Populus sp.
19
-
 quercetin mutant enzyme D257G, in 10 mM Tris/HCl buffer (pH 7.5), at 37°C Populus sp.
19
-
 kaempferol wild type enzyme, in 10 mM Tris/HCl buffer (pH 7.5), at 37°C Populus sp.
24
-
 quercetin wild type enzyme, in 10 mM Tris/HCl buffer (pH 7.5), at 37°C Populus sp.