Protein Variants | Comment | Organism |
---|---|---|
C139S | mutant retains 31% of the activity of the wild-type enzyme. While addition of free bicarbonate has almost no effect on the wild-type enzyme activity, the mutants enzyme is rescued by the addition of free bicarbonate. Catalytic efficiencies of the rescued mutant is 85% of wild-type activity | Escherichia coli |
E239A | catalytic efficiency is 0.2% of wild-type value | Escherichia coli |
E239Q | catalytic efficiency is less than 0.02% of wild-type value | Escherichia coli |
H266A | catalytic efficiency is 5.3% of wild-type value. While addition of free bicarbonate has almost no effect on the wild-type enzyme activity, the mutants enzyme is rescued by the addition of free bicarbonate. Catalytic efficiencies of the rescued mutant is 16% of wild-type activity | Escherichia coli |
Y317F | catalytic efficiency is 0.7% of wild-type value. While addition of free bicarbonate has almost no effect on the wild-type enzyme activity, the mutants enzyme is rescued by the addition of free bicarbonate. Catalytic efficiencies of the rescued mutant is 14% of wild-type activity | Escherichia coli |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.07 | - |
S-adenosyl-L-methionine | 37°C, pH 8.0, wild-type enzyme | Escherichia coli | |
0.075 | - |
S-adenosyl-L-methionine | 37°C, pH 8.0, mutant enzyme C139S | Escherichia coli | |
0.078 | - |
S-adenosyl-L-methionine | 37°C, pH 8.0, mutant enzyme H266A | Escherichia coli |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Escherichia coli | - |
- |
- |
Purification (Comment) | Organism |
---|---|
wild-type and six-histidine-tagged mutant enzymes H266A, Y317F, E239A, and E239Q | Escherichia coli |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
S-adenosyl-L-methionine + phospholipid olefinic fatty acid | - |
Escherichia coli | S-adenosyl-L-homocysteine + phospholipid cyclopropane fatty acid | - |
? |
Synonyms | Comment | Organism |
---|---|---|
cyclopropane fatty acid synthase | - |
Escherichia coli |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0025 | - |
S-adenosyl-L-methionine | 37°C, pH 8.0, mutant enzyme H266A | Escherichia coli | |
0.0142 | - |
S-adenosyl-L-methionine | 37°C, pH 8.0, mutant enzyme C139S | Escherichia coli | |
0.042 | - |
S-adenosyl-L-methionine | 37°C, pH 8.0, wild-type enzyme | Escherichia coli |