Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli BL21(DE3) cells | Cicer arietinum |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.007 | - |
S-adenosyl-L-methionine | in HEPES buffer, pH 7.5, at 37°C | Cicer arietinum | |
0.25 | - |
L-Val-L-Tyr-L-Pro-(L-iso-Asp)-L-His-L-Ala | in HEPES buffer, pH 7.5, at 37°C | Cicer arietinum |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Cicer arietinum | D2IWA7 | cultivar BGD72 | - |
Purification (Comment) | Organism |
---|---|
nickel-charged affnity column chromatography | Cicer arietinum |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
leaf | - |
Cicer arietinum | - |
root | - |
Cicer arietinum | - |
seed | PIMT1 is distributed across the organs with maximum levels in seed | Cicer arietinum | - |
shoot | - |
Cicer arietinum | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
S-adenosyl-L-methionine + L-Val-L-Tyr-L-Pro-(L-iso-Asp)-L-His-L-Ala | - |
Cicer arietinum | S-adenosyl-L-homocysteine + L-Val-L-Tyr-L-Pro-(L-iso-Asp alpha methyl ester)-L-His-L-Ala | - |
? |
Synonyms | Comment | Organism |
---|---|---|
PIMT1 | - |
Cicer arietinum |
protein L-isoaspartyl methyltransferase1 | - |
Cicer arietinum |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
50 | - |
- |
Cicer arietinum |
Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
---|---|---|---|
35 | 55 | - |
Cicer arietinum |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
60 | - |
at temperature above 60°C, PIMT activity shows a sharp decline | Cicer arietinum |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
9 | - |
- |
Cicer arietinum |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
8 | 10 | - |
Cicer arietinum |
pH Stability | pH Stability Maximum | Comment | Organism |
---|---|---|---|
9.5 | - |
above pH 9.5 the enzyme activity rapidly declines | Cicer arietinum |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
S-adenosyl-L-methionine | - |
Cicer arietinum |
Organism | Comment | Expression |
---|---|---|
Cicer arietinum | the activity is elevated about 3fold towards high temperature, cold, salt, dehydration, and oxidative stress induced by paraquat, as well as high pH conditions | up |
General Information | Comment | Organism |
---|---|---|
physiological function | PIMT1 may have a specifc role in stress adaptation apart from seed | Cicer arietinum |