Literature summary for 2.1.1.77 extracted from

Furuchi, T.; Kosugi, S.; Ohno, K.; Egawa, T.; Sekine, M.; Katane, M.; Homma, H.
High-performance liquid chromatographic method to measure protein L-isoaspartyl/D-aspartyl O-methyltransferase activity in cell lysates (2009), Anal. Biochem., 384, 207-212.

Search for more literature for 2.1.1.77

Cloned(Commentary)

Cloned (Comment)	Organism
His-tagged enzyme is expressed in Escherichia coli	Homo sapiens

KM Value [mM]

KM Value [mM]	KM Value Maximum [mM]	Substrate	Comment	Organism	Structure
0.055	-	7-nitro-2,1,3-benzoxadiazole-delta sleep-inducing peptide -L-isoaspartate	in 0.1 M sodium phosphate (pH 6.8), 1 mM EGTA, 0.004% (w/v) sodium azide, and 0.16% (v/v) Triton X-100, at 37°C	Homo sapiens

Organism

Organism	UniProt	Comment	Textmining
Homo sapiens	-	-	-

Purification (Commentary)

Purification (Comment)	Organism
nickel column affinity chromatography	Homo sapiens

Source Tissue

Source Tissue	Comment	Organism	Textmining
HEK-293 cell	-	Homo sapiens	-

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
S-adenosyl-L-methionine + 7-nitro-2,1,3-benzoxadiazole-delta sleep-inducing peptide-L-isoaspartate	-	Homo sapiens	S-adenosyl-L-homocysteine + 7-nitro-2,1,3-benzoxadiazole-delta sleep-inducing peptide-L-isoaspartate alpha-methyl ester	-	?
S-adenosyl-L-methionine + protein L-isoaspartate	-	Homo sapiens	S-adenosyl-L-homocysteine + protein L-isoaspartate alpha-methyl ester	-	?

Synonyms

Synonyms	Comment	Organism
PIMT	-	Homo sapiens
protein L-isoaspartyl/D-aspartyl O-methyltransferase	-	Homo sapiens

Cofactor

Cofactor	Comment	Organism	Structure
S-adenosyl-L-methionine	-	Homo sapiens

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Release 2023.1 (January 2023)