Activating Compound | Comment | Organism | Structure |
---|---|---|---|
2-mercaptoethanol | relative activity 105% | Chrysosplenium americanum | |
iodoacetamide | relative activity 105% | Chrysosplenium americanum |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
N-ethylmaleimide | - |
Chrysosplenium americanum | |
p-chloromercuribenzoate | - |
Chrysosplenium americanum |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
K+ | relative activity 115% | Chrysosplenium americanum | |
Mn2+ | relative activity 110% | Chrysosplenium americanum |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
57000 | - |
gel filtration | Chrysosplenium americanum |
65000 | - |
gel filtration | Chrysosplenium americanum |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
S-adenosyl-L-methionine + 3,5,7,3',4'-pentahydroxyflavone | Citrofortunella microcarpa | - |
S-adenosyl-L-homocysteine + 3-methoxy-5,7,3',4'-tetrahydroxyflavone | - |
? | |
S-adenosyl-L-methionine + 3,5,7,3',4'-pentahydroxyflavone | Chrysosplenium americanum | - |
S-adenosyl-L-homocysteine + 3-methoxy-5,7,3',4'-tetrahydroxyflavone | 3-O-methylquercetin | ? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Chrysosplenium americanum | - |
- |
- |
Citrofortunella microcarpa | - |
calamondin orange, synonym Citrofortunella mitis | - |
Purification (Comment) | Organism |
---|---|
partially | Chrysosplenium americanum |
partially | Citrofortunella microcarpa |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
additional information | - |
53 pkat/mg | Chrysosplenium americanum |
Storage Stability | Organism |
---|---|
-15°C, purified enzyme, 0.2 M potassium phosphate, pH 8.0, 20% glycerol, 5 mM dithiothreitol, stable for several months | Chrysosplenium americanum |
4°C, partially purified enzyme loses 80% of its activity over a period of 4-6 weeks | Chrysosplenium americanum |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
S-adenosyl-L-methionine + 3,5,7,3',4'-pentahydroxyflavone | - |
Chrysosplenium americanum | S-adenosyl-L-homocysteine + 3-methoxy-5,7,3',4'-tetrahydroxyflavone | - |
? | |
S-adenosyl-L-methionine + 3,5,7,3',4'-pentahydroxyflavone | - |
Citrofortunella microcarpa | S-adenosyl-L-homocysteine + 3-methoxy-5,7,3',4'-tetrahydroxyflavone | - |
? | |
S-adenosyl-L-methionine + 3,5,7,3',4'-pentahydroxyflavone | - |
Chrysosplenium americanum | S-adenosyl-L-homocysteine + 3-methoxy-5,7,3',4'-tetrahydroxyflavone | 3-O-methylquercetin | ? | |
S-adenosyl-L-methionine + 7-methylquercetin | i.e. rhamnetin, 30% of the activity with quercetin | Chrysosplenium americanum | ? | - |
? |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.8 | 8.2 | - |
Chrysosplenium americanum |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
7.2 | 9.3 | about 50% of activity maximum at pH 7.2 and 9.3 | Chrysosplenium americanum |