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Literature summary for 2.1.1.74 extracted from

  • Cicmil, N.
    Crystallization and preliminary X-ray crystallographic characterization of TrmFO, a folate-dependent tRNA methyltransferase from Thermotoga maritima (2008), Acta Crystallogr. Sect. F, 64, 193-195.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

Cloned (Comment) Organism
overexpression in Escherichia coli strain BL21(DE3) Thermotoga maritima

Crystallization (Commentary)

Crystallization (Comment) Organism
purified recombinant enzyme, from 25% v/v PEG 4000, 100 mM NaCl and sodium citrate buffer, pH 5.0, at 291 K using the hanging-drop vapor-diffusion method in 0.0016 ml with 8 mg/ml protein, 7 days, X-ray diffraction structure determination and analysis at 2.6 A resolution Thermotoga maritima

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
5,10-methylenetetrahydrofolate + tRNA containing uridine at position 54 + FADH2 Thermotoga maritima TrmFO is a methyltransferase that catalyzes the formation of 5-methyluridine or ribothymidine at position 54 in tRNA in some Gram-positive bacteria tetrahydrofolate + tRNA containing ribothymidine at position 54 + FAD
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?

Organism

Organism UniProt Comment Textmining
Thermotoga maritima
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gene trmFO
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Purification (Commentary)

Purification (Comment) Organism
recombinant enzyme from Escherichia coli strain BL21(DE3) by anion and cation exchange chromatography and gel filtration to over 95% purity Thermotoga maritima

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
5,10-methylenetetrahydrofolate + tRNA containing uridine at position 54 + FADH2 TrmFO is a methyltransferase that catalyzes the formation of 5-methyluridine or ribothymidine at position 54 in tRNA in some Gram-positive bacteria Thermotoga maritima tetrahydrofolate + tRNA containing ribothymidine at position 54 + FAD
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?
N5,N10-methylenetetrahydrofolate + tRNA containing uridine at position 54 + FADH2
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Thermotoga maritima tetrahydrofolate + tRNA containing ribothymidine at position 54 + FAD
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?

Synonyms

Synonyms Comment Organism
TRMFO
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Thermotoga maritima

Cofactor

Cofactor Comment Organism Structure
FAD dependent on Thermotoga maritima