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Literature summary for 2.1.1.72 extracted from
- Proximal recognition sites facilitate intrasite hopping by DNA adenine methyltransferase: Mechanistic exploration of epigenetic gene regulation (2012), J. Biol. Chem., 287, 22873-22881.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| expressed in Escherichia coli XL2Blue cells | Escherichia coli |
Protein Variants
| Protein Variants | Comment | Organism |
|---|---|---|
| N126A | the mutant shows large reduction in methylation activity | Escherichia coli |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Escherichia coli | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| phosphocellulose column chromatography and Blue Sepharose column chromatography | Escherichia coli |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| S-adenosyl-L-methionine + DNA adenine | the enzyme methylates adenines at the N6 position of palindromic 5-GATC-3 sites. The enzyme ethylates both strands of the same site prior to fully dissociating from the DNA, a process referred to as intrasite processivity. Intrasite processivity is disrupted when the DNA flanking a single GATC site is longer than 400 bp on either side. The introduction of a second GATC site within this flanking DNA reinstates intrasite methylation of both sites | Escherichia coli | S-adenosyl-L-homocysteine + DNA 6-methyladenine | - |
? |  |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| Dam | - |
Escherichia coli |
| DNA adenine methyltransferase | - |
Escherichia coli |
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Release 2023.1 (January 2023)
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