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Literature summary for 2.1.1.72 extracted from

  • Erova, T.E.; Fadl, A.A.; Sha, J.; Khajanchi, B.K.; Pillai, L.L.; Kozlova, E.V.; Chopra, A.K.
    Mutations within the catalytic motif of DNA adenine methyltransferase (Dam) of Aeromonas hydrophila cause the virulence of the Dam-overproducing strain to revert to that of the wild-type phenotype (2006), Infect. Immun., 74, 5763-5772.
    View publication on PubMedView publication on EuropePMC
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Application

Application Comment Organism
medicine methyltransferase activity associated with Dam is essential for attenuation of Aeromonas hydrophila virulence, immunization of mice with a native-Dam-overproducing strain at the same 50% lethal dose does not result in any lethality and provides protection to animals after subsequent challenge with a lethal dose of the control strain Aeromonas hydrophila

Cloned(Commentary)

Cloned (Comment) Organism
native or mutated dam gene overexpressed from the pBAD/damAhSSU pBAD/damD/A, or pBAD/damY/A plasmid under the control of an araBAD promoter, transformed to Escherichia coli GM33 Aeromonas hydrophila

Protein Variants

Protein Variants Comment Organism
D71A phenotype of native Dam Aeromonas hydrophila
E76A phenotype of native Dam Aeromonas hydrophila
additional information mutated dam gene, in which either the aspartic acid or the tyrosine residue was changed to alanine, do not possess MTase activity in the DPPY motif, resistant to DpnI digestion and sensitive to DpnII restriction endonuclease cutting, overproduction in Aeromonas hydrophila results in bacterial motility, hemolytic and cytotoxic activities associated with the cytotoxic enterotoxin, and protease activity similar to that of the wild-type Aeromonas hydrophila

Organism

Organism UniProt Comment Textmining
Aeromonas hydrophila
-
-
-
Aeromonas hydrophila SSU
-
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
S-adenosyl-L-methionine + DNA adenine
-
 Aeromonas hydrophila S-adenosyl-L-homocysteine + DNA 6-methyladenine
-
 ?
S-adenosyl-L-methionine + DNA adenine
-
 Aeromonas hydrophila SSU S-adenosyl-L-homocysteine + DNA 6-methyladenine
-
 ?

Synonyms

Synonyms Comment Organism
Dam
-
 Aeromonas hydrophila
DNA adenine methyltransferase
-
 Aeromonas hydrophila