KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
additional information | - |
additional information | steady-state and pre-steady-state kinetics, single turnover methylation kinetics | Tequatrovirus T4 |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
S-adenosyl-L-methionine + DNA adenine | Tequatrovirus T4 | unmethylated or hemimethylated DNA | S-adenosyl-L-homocysteine + DNA 6-methylaminopurine | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Tequatrovirus T4 | - |
- |
- |
Reaction | Comment | Organism | Reaction ID |
---|---|---|---|
S-adenosyl-L-methionine + adenine in DNA = S-adenosyl-L-homocysteine + N6-methyladenine in DNA | two distinct stages of methylation under single turnover conditions | Tequatrovirus T4 |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | enzyme and S-adenosyl-L-methionine form a catalytically active complex | Tequatrovirus T4 | ? | - |
? | |
S-adenosyl-L-methionine + (CGG*CCG)73 oligodeoxynucleotide duplexes 5'-GATC/5'-GATC | - |
Tequatrovirus T4 | ? | - |
? | |
S-adenosyl-L-methionine + DNA adenine | unmethylated or hemimethylated DNA | Tequatrovirus T4 | S-adenosyl-L-homocysteine + DNA 6-methylaminopurine | - |
? | |
S-adenosyl-L-methionine + DNA adenine | unmethylated or hemimethylated DNA, two distinct stages of methylation under single turnover conditions | Tequatrovirus T4 | S-adenosyl-L-homocysteine + DNA 6-methylaminopurine | - |
? | |
S-adenosyl-L-methionine + oligodeoxynucleotide duplexes | substrate contains one single specific recognition site as 5'-GATC/5'-GATC, or modified variants | Tequatrovirus T4 | S-adenosyl-L-homocysteine + DNA 6-methylaminopurine | - |
? |
Subunits | Comment | Organism |
---|---|---|
monomer | enzyme and S-adenosyl-L-methionine form a catalytically active complex | Tequatrovirus T4 |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
25 | - |
assay at | Tequatrovirus T4 |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
additional information | - |
additional information | - |
Tequatrovirus T4 | |
0.21 | - |
oligodeoxynucleotide duplexes 5'-GATC/5'-GATC | - |
Tequatrovirus T4 |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
8 | - |
assay at | Tequatrovirus T4 |