Inhibitors | Comment | Organism | Structure |
---|---|---|---|
20mer fully methylated DNA duplex | product inhibition, noncompetitive against both S-adenosyl-L-methionine and 20mer unmethylated DNA duplex | Bacillus amyloliquefaciens | |
additional information | no substrate inhibition even at high concentrations, no formation of dead-end complexes | Bacillus amyloliquefaciens | |
S-adenosyl-L-homocysteine | product inhibition, competitive against S-adenosyl-L-methionine, noncompetitive to 20mer unmethylated DNA duplex | Bacillus amyloliquefaciens |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
additional information | - |
additional information | detailed kinetics, enzyme shows a simple kinetic behaviour towards a 20mer duplex DNA, random bi bi mechanism | Bacillus amyloliquefaciens |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
S-adenosyl-L-methionine + DNA adenine | Bacillus amyloliquefaciens | - |
S-adenosyl-L-homocysteine + DNA 6-methylaminopurine | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Bacillus amyloliquefaciens | - |
- |
- |
Reaction | Comment | Organism | Reaction ID |
---|---|---|---|
S-adenosyl-L-methionine + adenine in DNA = S-adenosyl-L-homocysteine + N6-methyladenine in DNA | reaction scheme and random bi bi kinetic mechanism | Bacillus amyloliquefaciens |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | the common methyl transfer from S-adenosyl-L-methionine to an exocyclic amino group, catalyzed by many enzymes, does not dictate a common kinetic scheme for MTases, comparison to T4Dam MTase from bacteriophage T4 | Bacillus amyloliquefaciens | ? | - |
? | |
S-adenosyl-L-methionine + DNA adenine | - |
Bacillus amyloliquefaciens | S-adenosyl-L-homocysteine + DNA 6-methylaminopurine | - |
? |
Synonyms | Comment | Organism |
---|---|---|
BamHI MTase | - |
Bacillus amyloliquefaciens |
DNA-(adenine-N6-)-methyltransferase | - |
Bacillus amyloliquefaciens |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
37 | - |
assay at | Bacillus amyloliquefaciens |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
8 | - |
assay at | Bacillus amyloliquefaciens |
Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
---|---|---|---|---|---|
additional information | - |
additional information | detailed inhibition kinetics | Bacillus amyloliquefaciens |