Cloned (Comment) | Organism |
---|---|
expression in Escherichia coli. Transgenic Festuca arundinacea plants carrying either sense or antisense COMT gene constructs are obtained by microprojectile bombardment of single-genotype-derived embryogenic suspension cells. Two co-suppressed transgenic lines are down-regulated in their lignin biosynthesis. These down-regulated transgenic plants show substantially reduced levels of transcripts, significantly reduced enzymatic activities, significantly decreased lignin content, apparently altered lignin composition and significantly increased digestibility | Lolium arundinaceum |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0109 | - |
5-hydroxyferulic acid | pH 7.5, 30°C | Lolium arundinaceum | |
0.0148 | - |
caffeoyl aldehyde | pH 7.5, 30°C | Lolium arundinaceum | |
0.0185 | - |
5-hydroxyconiferaldehyde | pH 7.5, 30°C | Lolium arundinaceum | |
0.027 | - |
caffeoyl alcohol | pH 7.5, 30°C | Lolium arundinaceum | |
0.0497 | - |
caffeic acid | pH 7.5, 30°C | Lolium arundinaceum | |
0.068 | - |
5-hydroxyconiferyl alcohol | pH 7.5, 30°C | Lolium arundinaceum |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
S-adenosyl-L-methionine + 3,4,5-trihydroxycinnamic acid | Lolium arundinaceum | key lignin biosynthetic enzyme. Down-regulated transgenic plants show substantially reduced levels of transcripts, significantly reduced enzymatic activities, significantly decreased lignin content, apparently altered lignin composition and significantly increased digestibility | S-adenosyl-L-homocysteine + 4,5-dihydroxy-3-methoxycinnamic acid | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Lolium arundinaceum | - |
- |
- |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
S-adenosyl-L-methionine + 3,4,5-trihydroxycinnamic acid | - |
Lolium arundinaceum | S-adenosyl-L-homocysteine + 4,5-dihydroxy-3-methoxycinnamic acid | - |
? | |
S-adenosyl-L-methionine + 3,4,5-trihydroxycinnamic acid | key lignin biosynthetic enzyme. Down-regulated transgenic plants show substantially reduced levels of transcripts, significantly reduced enzymatic activities, significantly decreased lignin content, apparently altered lignin composition and significantly increased digestibility | Lolium arundinaceum | S-adenosyl-L-homocysteine + 4,5-dihydroxy-3-methoxycinnamic acid | - |
? | |
S-adenosyl-L-methionine + 5-hydroxyconiferaldehyde | - |
Lolium arundinaceum | S-adenosyl-L-homocysteine + 5-methoxyconiferaldehyde | - |
? | |
S-adenosyl-L-methionine + 5-hydroxyconiferyl alcohol | - |
Lolium arundinaceum | S-adenosyl-L-homocysteine + ? | - |
? | |
S-adenosyl-L-methionine + 5-hydroxyferulic acid | - |
Lolium arundinaceum | S-adenosyl-L-homocysteine + sinapic acid | - |
? | |
S-adenosyl-L-methionine + caffeic acid | - |
Lolium arundinaceum | S-adenosyl-L-homocysteine + ferulic acid | - |
r | |
S-adenosyl-L-methionine + caffeoyl alcohol | - |
Lolium arundinaceum | S-adenosyl-L-homocysteine + coniferyl alcohol | - |
? | |
S-adenosyl-L-methionine + caffeoyl aldehyde | - |
Lolium arundinaceum | S-adenosyl-L-homocysteine + ferulic aldehyde | - |
? |
Synonyms | Comment | Organism |
---|---|---|
COMT | - |
Lolium arundinaceum |