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Literature summary for 2.1.1.68 extracted from

  • Poulton, J.E.; Hahlbrock, K.; Grisebach, H.
    Enzymic synthesis of lignin precursors. Purification of properties of the S-adenosyl-l-methionine: caffeic acid 3-O-methyltransferase from soybean cell suspension cultures (1976), Arch. Biochem. Biophys., 176, 449-456.
    View publication on PubMed
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Inhibitors

Inhibitors Comment Organism Structure
luteolin
-
 Glycine max
additional information no inhibition by EDTA Glycine max
quercetin
-
 Glycine max
S-adenosyl-L-homocysteine strong, product inhibition, competitive Glycine max

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.015
-
 S-adenosyl-L-methionine
-
 Glycine max
0.05
-
 Protocatechuic aldehyde
-
 Glycine max
0.055
-
 5-hydroxyferulic acid
-
 Glycine max
0.1
-
 3,4,5-trihydroxycinnamic acid
-
 Glycine max
0.133
-
 caffeic acid
-
 Glycine max

Organism

Organism UniProt Comment Textmining
Glycine max
-
 var. Mandarin, soybean
-

Reaction

Reaction Comment Organism Reaction ID
S-adenosyl-L-methionine + 3,4-dihydroxy-trans-cinnamate = S-adenosyl-L-homocysteine + 3-methoxy-4-hydroxy-trans-cinnamate mechanism Glycine max
a

Source Tissue

Source Tissue Comment Organism Textmining
cell suspension culture
-
 Glycine max
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
0.00546
-
-
 Glycine max

Storage Stability

Storage Stability Organism
4°C, 0.02% NaN3, at least 2 months Glycine max

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
additional information methylation only in meta-position Glycine max ?
-
 ?
S-adenosyl-L-methionine + 3,4,5-trihydroxycinnamic acid
-
 Glycine max S-adenosyl-L-homocysteine + 4,5-dihydroxy-3-methoxycinnamic acid
-
 ?
S-adenosyl-L-methionine + 3,4-dihydroxy-trans-cinnamate
-
 Glycine max S-adenosyl-L-homocysteine + 3-methoxy-4-hydroxy-trans-cinnamate i.e. ferulate ?
S-adenosyl-L-methionine + 5-hydroxyferulic acid
-
 Glycine max S-adenosyl-L-homocysteine + sinapic acid
-
 ?
S-adenosyl-L-methionine + catechol poor substrate Glycine max S-adenosyl-L-homocysteine + 2-methoxyphenol
-
 ?
S-adenosyl-L-methionine + protocatechuic acid poor substrate Glycine max S-adenosyl-L-homocysteine + 4-hydroxy-3-methoxybenzoate
-
 ?
S-adenosyl-L-methionine + protocatechuic aldehyde
-
 Glycine max S-adenosyl-L-homocysteine + 4-hydroxy-3-methoxybenzaldehyde
-
 ?

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
6.5 7 methylation of caffeic and 5-hydroxyferulic acid Glycine max

pH Range

pH Minimum pH Maximum Comment Organism
5 8.5 more than half-maximal activity at pH 5.0 and 8.5 Glycine max

Ki Value [mM]

Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
0.0069
-
 S-adenosyl-L-homocysteine
-
 Glycine max