General Stability | Organism |
---|---|
2-mercaptoethanol stabilizes | Nicotiana tabacum |
freezing causes complete inactivation | Nicotiana tabacum |
gel filtration leads to severe loss of activity | Nicotiana tabacum |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0045 | - |
S-adenosyl-L-methionine | - |
Nicotiana tabacum | |
0.03 | - |
esculetin | isozyme I, m- and p-methylation | Nicotiana tabacum | |
0.04 | - |
homocatechol | isozyme I | Nicotiana tabacum | |
0.065 | - |
5-hydroxyferulic acid | - |
Nicotiana tabacum | |
0.1 | - |
caffeic acid | isozyme I | Nicotiana tabacum | |
0.23 | - |
Hydrocaffeic acid | isozyme I | Nicotiana tabacum | |
0.7 | - |
Protocatechuic aldehyde | isozyme I, m- and p-methylation | Nicotiana tabacum | |
2 | - |
catechol | isozyme I | Nicotiana tabacum | |
2.5 | - |
chlorogenic acid | isozyme I | Nicotiana tabacum | |
2.5 | - |
protocatechuic acid | isozyme I, m- and p-methylation | Nicotiana tabacum |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
additional information | no Mg2+ requirement | Nicotiana tabacum |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
93000 | - |
isozyme I, gel filtration | Nicotiana tabacum |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
S-adenosyl-L-methionine + 3,4-dihydroxy-trans-cinnamate | Nicotiana tabacum | i.e. caffeic acid, involved in the biosynthesis of lignin | S-adenosyl-L-homocysteine + 3-methoxy-4-hydroxy-trans-cinnamate | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Nicotiana tabacum | - |
tobacco, strain Samsun NN, three isozymes isozyme I, isozyme II and isozyme III, only isozyme I has the characteristics of caffeate O-methyltransferase | - |
Purification (Comment) | Organism |
---|---|
three caffeic acid methylating activities: isozymes I, II, III, with an enzyme density of 1.277 kg/l, determined by isopycnic equilibrium centrifugation | Nicotiana tabacum |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
leaf | highly infected by tobacco mosaic virus | Nicotiana tabacum | - |
Storage Stability | Organism |
---|---|
0°C, 65%-85% of initial activity retained after 5 months in the presence of 2-mercaptoethanol | Nicotiana tabacum |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | p- and m-methylation of protocatechuic aldehyde and acid, as well as esculetin | Nicotiana tabacum | ? | - |
? | |
S-adenosyl-L-methionine + 3,4-dihydroxy-hydrocinnamic acid | - |
Nicotiana tabacum | S-adenosyl-L-homocysteine + 4-hydroxy-3-methoxyhydrocinnamic acid | - |
? | |
S-adenosyl-L-methionine + 3,4-dihydroxy-trans-cinnamate | i.e. caffeate, m-specific methylation | Nicotiana tabacum | S-adenosyl-L-homocysteine + 3-methoxy-4-hydroxy-trans-cinnamate | - |
? | |
S-adenosyl-L-methionine + 3,4-dihydroxy-trans-cinnamate | i.e. caffeic acid, involved in the biosynthesis of lignin | Nicotiana tabacum | S-adenosyl-L-homocysteine + 3-methoxy-4-hydroxy-trans-cinnamate | - |
? | |
S-adenosyl-L-methionine + 3-(3,4-dihydroxyphenyl)propanoic acid | - |
Nicotiana tabacum | S-adenosyl-L-homocysteine + 3-(4-hydroxy-3-methoxyphenyl)propanoic acid | - |
? | |
S-adenosyl-L-methionine + 5-hydroxyferulic acid | best substrate | Nicotiana tabacum | S-adenosyl-L-homocysteine + sinapic acid | - |
? | |
S-adenosyl-L-methionine + 5-hydroxyferulic acid | m-specific methylation | Nicotiana tabacum | S-adenosyl-L-homocysteine + sinapic acid | - |
? | |
S-adenosyl-L-methionine + catechol | isozyme II and III, no substrate for isozyme I | Nicotiana tabacum | S-adenosyl-L-homocysteine + 2-methoxyphenol | - |
? | |
S-adenosyl-L-methionine + chlorogenic acid | - |
Nicotiana tabacum | ? | - |
? | |
S-adenosyl-L-methionine + esculetin | - |
Nicotiana tabacum | ? | - |
? | |
S-adenosyl-L-methionine + homocatechol | - |
Nicotiana tabacum | S-adenosyl-L-homocysteine + 2-methoxy-4-methylphenol | - |
? | |
S-adenosyl-L-methionine + protocatechuic acid | - |
Nicotiana tabacum | S-adenosyl-L-homocysteine + 4-hydroxy-3-methoxybenzoate | - |
? | |
S-adenosyl-L-methionine + protocatechuic aldehyde | - |
Nicotiana tabacum | S-adenosyl-L-homocysteine + 4-hydroxy-3-methoxybenzaldehyde | - |
? |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
45 | - |
isozyme I inactivates after 5 min, isozyme II loses 70% of activity after 2 h, isozyme III has 50% activation after 2 h | Nicotiana tabacum |