Inhibitors | Comment | Organism | Structure |
---|---|---|---|
S-adenosyl-L-homocysteine | strong, product inhibition, competitive | Medicago sativa |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0041 | - |
S-adenosyl-L-methionine | - |
Medicago sativa | |
0.058 | - |
caffeic acid | - |
Medicago sativa |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
103000 | - |
gel filtration | Medicago sativa |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
S-adenosyl-L-methionine + 3,4-dihydroxy-trans-cinnamate | Medicago sativa | i.e. caffeic acid, involved in the biosynthesis of lignin | S-adenosyl-L-homocysteine + 3-methoxy-4-hydroxy-trans-cinnamate | - |
? | |
S-adenosyl-L-methionine + 3,4-dihydroxy-trans-cinnamate | Medicago sativa Saranac | i.e. caffeic acid, involved in the biosynthesis of lignin | S-adenosyl-L-homocysteine + 3-methoxy-4-hydroxy-trans-cinnamate | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Medicago sativa | - |
alfalfa | - |
Medicago sativa Saranac | - |
alfalfa | - |
Purification (Comment) | Organism |
---|---|
affinity chromatography | Medicago sativa |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
root nodule | - |
Medicago sativa | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
0.017 | - |
- |
Medicago sativa |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
S-adenosyl-L-methionine + 3,4-dihydroxy-trans-cinnamate | - |
Medicago sativa | S-adenosyl-L-homocysteine + 3-methoxy-4-hydroxy-trans-cinnamate | i.e. ferulate | ? | |
S-adenosyl-L-methionine + 3,4-dihydroxy-trans-cinnamate | i.e. caffeic acid, involved in the biosynthesis of lignin | Medicago sativa | S-adenosyl-L-homocysteine + 3-methoxy-4-hydroxy-trans-cinnamate | - |
? | |
S-adenosyl-L-methionine + 3,4-dihydroxy-trans-cinnamate | - |
Medicago sativa Saranac | S-adenosyl-L-homocysteine + 3-methoxy-4-hydroxy-trans-cinnamate | i.e. ferulate | ? | |
S-adenosyl-L-methionine + 3,4-dihydroxy-trans-cinnamate | i.e. caffeic acid, involved in the biosynthesis of lignin | Medicago sativa Saranac | S-adenosyl-L-homocysteine + 3-methoxy-4-hydroxy-trans-cinnamate | - |
? | |
S-adenosyl-L-methionine + 5-hydroxyferulic acid | at 70% the rate of caffeic acid methylation | Medicago sativa | S-adenosyl-L-homocysteine + sinapic acid | - |
? | |
S-adenosyl-L-methionine + 5-hydroxyferulic acid | at 70% the rate of caffeic acid methylation | Medicago sativa Saranac | S-adenosyl-L-homocysteine + sinapic acid | - |
? | |
S-adenosyl-L-methionine + daidzein | poor substrate | Medicago sativa | ? | - |
? | |
S-adenosyl-L-methionine + daidzein | poor substrate | Medicago sativa Saranac | ? | - |
? | |
S-adenosyl-L-methionine + genistein | poor substrate | Medicago sativa | ? | - |
? | |
S-adenosyl-L-methionine + protocatechuic acid | poor substrate | Medicago sativa | S-adenosyl-L-homocysteine + 4-hydroxy-3-methoxybenzoate | - |
? | |
S-adenosyl-L-methionine + protocatechuic acid | poor substrate | Medicago sativa Saranac | S-adenosyl-L-homocysteine + 4-hydroxy-3-methoxybenzoate | - |
? | |
S-adenosyl-L-methionine + pyrogallol | poor substrate | Medicago sativa | ? | - |
? |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7 | - |
around | Medicago sativa |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
6.5 | 8 | more than half-maximal activity at pH 6.5 and 8.0 | Medicago sativa |
Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
---|---|---|---|---|---|
0.00044 | - |
S-adenosyl-L-homocysteine | - |
Medicago sativa |