Organism | UniProt | Comment | Textmining |
---|---|---|---|
Homo sapiens | P16455 | - |
- |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
DNA containing 6-O-methylguanine + [protein]-L-cysteine | - |
Homo sapiens | DNA lacking 6-O-methylguanine + [protein]-S-methyl-L-cysteine | - |
? | |
additional information | in the catalytic demethylation of DNA containing 6-O-methylguanine, roles of six amino acids, i.e., Cys145, His146, Glu172, Tyr114, Lys165, and Ser159 are involved. At the first step, Cys145 in the Cys145-water-His146-Glu172 tetrad is converted to cysteine thiolate anion while at the second step, abstraction of the Tyr114 proton by the N3 site of DNA containing 6-O-methylguanine occurs in a barrierless manner. In the third step, abstraction of Lys165 proton by deprotonated Tyr114 and transfer of the methyl group of DNA containing 6-O-methylguanine to the thiolate group of Cys145 anion occur simultaneously. | Homo sapiens | ? | - |
? |
Synonyms | Comment | Organism |
---|---|---|
AGT | - |
Homo sapiens |
MGMT | - |
Homo sapiens |
O6-alkylguanine-DNA alkyltransferase | - |
Homo sapiens |
O6-methylguanine-DNA methyltransferase | - |
Homo sapiens |