Cloned (Comment) | Organism |
---|---|
ligated into the pHybLex/Zeo plasmid and electroporated into Escherichia coli XL1-Blue, yielding a library of at least 320000 independent clones, genes of selected AGT mutants subcloned into the pGEX-2T plasmid, allowing for the expression of the AGT mutants as GST fusion proteins | Homo sapiens |
Protein Variants | Comment | Organism |
---|---|---|
A154T | ca. 4fold increased activity, AGT mutant selected using phage display | Homo sapiens |
A41D/S115T/I151N | 1.6fold increased activity, AGT mutant selected using yeast three-hybrid system | Homo sapiens |
D42E/A51T/A64V/K104M | 0.5fold increased activity, AGT mutant selected using yeast three-hybrid system | Homo sapiens |
D42E/P47L/V155L/K178M | 1.9fold increased activity, AGT mutant selected using yeast three-hybrid system | Homo sapiens |
E110D/L120M | 0.7fold increased activity, AGT mutant selected using yeast three-hybrid system | Homo sapiens |
E25K | 0.7fold increased activity, AGT mutant selected using yeast three-hybrid system | Homo sapiens |
E92D/I151V/R175W | 1.0fold increased activity, AGT mutant selected using yeast three-hybrid system | Homo sapiens |
F79I/V88I/F89L | 0.7fold increased activity, AGT mutant selected using yeast three-hybrid system | Homo sapiens |
G122C | 0.9fold increased activity, AGT mutant selected using yeast three-hybrid system | Homo sapiens |
H71Y/A154T | 3.3fold increased activity, AGT mutant selected using phage display | Homo sapiens |
K104E/T127A/A154T | 4.5fold increased activity, AGT mutant selected using phage display | Homo sapiens |
K107R/A154T | 4.7fold increased activity, AGT mutant selected using phage display | Homo sapiens |
K8R/K104E/I151T | 1.4fold increased activity, AGT mutant selected using phage display | Homo sapiens |
K8T/A51T/I112V/A154T | 5.5fold increased activity, AGT mutant selected using phage display | Homo sapiens |
K8T/T127A/A154T/H174R | 5.8fold increased activity, AGT mutant selected using phage display | Homo sapiens |
L33F/A68T | 2.1fold increased activity, AGT mutant selected using yeast three-hybrid system | Homo sapiens |
L33F/N123Y | 2.2fold increased activity, AGT mutant selected using yeast three-hybrid system | Homo sapiens |
L33F/V44A/V52A/A154T | 6.1fold increased activity, AGT mutant selected using phage display | Homo sapiens |
L66M/K131R | 1.5fold increased activity, AGT mutant selected using yeast three-hybrid system | Homo sapiens |
M1V/V164M | 1.9fold increased activity, AGT mutant selected using phage display | Homo sapiens |
additional information | MAGT decreased activity can be restored by combination of saturation mutagenesis of residues 150-154 and 31-35 from phage display and yeast three-hybrid system selections, reveals a mutant with 17fold higher activity than MAGT and a 52fold higher activity than wild-type AGT, is the most active AGT mutant against O6-benzylguanine derivatives described so far | Homo sapiens |
N123S | 1.3fold increased activity, AGT mutant selected using phage display | Homo sapiens |
N150D | 1.8fold increased activity, AGT mutant selected using phage display | Homo sapiens |
N157/S159/C62A/C150N/G131K/G132T/M134L/R135S/Q115S/Q116H/K125A/A127T/R128A/S151I/S152N | called MAGT with 15 different mutations in a single protein, has23fold increase in activity relative to wild-type, is resistant against N9-substituted BG derivatives used for inhibition of wild-type, shows suppressed affinity towards DNA | Homo sapiens |
Q90R/K101N/F108I/V164L | 1.2fold increased activity, AGT mutant selected using yeast three-hybrid system | Homo sapiens |
R175L | 0.6fold increased activity, AGT mutant selected using yeast three-hybrid system | Homo sapiens |
T11I/N67K/Q72L | 1.0fold increased activity, AGT mutant selected using yeast three-hybrid system | Homo sapiens |
T127A | 2.2fold increased activity, AGT mutant selected using phage display | Homo sapiens |
T38M/A41D/A64T/G173C | 0.8fold increased activity, AGT mutant selected using yeast three-hybrid system | Homo sapiens |
V149I/A154T | 4.5fold increased activity, AGT mutant selected using phage display | Homo sapiens |
V44G/V106A/I151T/A170T | 1.4fold increased activity, AGT mutant selected using yeast three-hybrid system | Homo sapiens |
V46A/A50V/P58V/A154T | 3.4fold increased activity, AGT mutant selected using phage display | Homo sapiens |
V52A/I151S/K178E | 2.3fold increased activity, AGT mutant selected using phage display | Homo sapiens |
V52I/V164M | 2.1fold increased activity, AGT mutant selected using yeast three-hybrid system | Homo sapiens |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
O6-benzylguanine | - |
Homo sapiens |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Homo sapiens | - |
- |
- |
Synonyms | Comment | Organism |
---|---|---|
AGT | - |
Homo sapiens |
O6-alkylguanine-DNA alkyltransferase | - |
Homo sapiens |