Application | Comment | Organism |
---|---|---|
medicine | temozolomide appears to be a good candidate for use in conjunction with hyperthermia for regional chemotherapy of melanoma | Homo sapiens |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
4-[bis(2-chloroethyl)amino]-L-phenylalanine | hyperthermia enhances the inhibitory effects of L-phenylalanine mustard on melanoma cell growth | Homo sapiens | |
temozolomide | TMZ; tumor cells with high levels of MGMT and/or with a defective DNA mismatch repair are resistant to temozolomide, hyperthermia significantly enhances temozolomide cytotoxicity in mismatch repair-proficient cells, either endowed or not with MGMT activity, and in mismatch repair-deficient cells, hyperthermia alone does not affect MGMT activity, but enhances the enzyme depletion induced by temozolomide treatment | Homo sapiens |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
DNA (containing 6-O-methylguanine) + [protein]-L-cysteine | Homo sapiens | - |
DNA (without 6-O-methylguanine) + [protein]-S-methyl-L-cysteine | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Homo sapiens | - |
- |
- |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
M14 cell | - |
Homo sapiens | - |
melanoma cell line | - |
Homo sapiens | - |
melanoma cell line | three lines with different MGMT activity and functional status of the mismatch repair system | Homo sapiens | - |
additional information | PR-Mel cell and GL-Mel-D2 cell | Homo sapiens | - |
PR-Mel cell | - |
Homo sapiens | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
additional information | - |
M14 cell line, 136, PR-Mel cell line 15 fmol of 3H-methyl groups transferred per mg of protein in cell extract | Homo sapiens |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
DNA (containing 6-O-methylguanine) + [protein]-L-cysteine | - |
Homo sapiens | DNA (without 6-O-methylguanine) + [protein]-S-methyl-L-cysteine | - |
? |
Synonyms | Comment | Organism |
---|---|---|
MGMT | - |
Homo sapiens |
O6-methylguanine-DNA methyltransferase | - |
Homo sapiens |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
37 | - |
- |
Homo sapiens |
41.5 | - |
hyperthermic condition | Homo sapiens |