Application | Comment | Organism |
---|---|---|
analysis | strategy of loop insertion to alter enzyme specificity shall be general and applicable to other classes of proteins, the isolated AGT mutant can be applied in molecular imaging, where the mutant and parental AGTs are used to label two different AGT fusion proteins with different fluorophores in the same living cell or in vitro, allows establishment of fluorescence-based assays to detect protein-protein interactions and measure enzymatic activities | Homo sapiens |
Protein Variants | Comment | Organism |
---|---|---|
G160W | cells overexpressing W160AGT do not become labeled, even when incubated with O6-propargylguanine for extended periods of time | Homo sapiens |
additional information | insertion of random amino acid loops into the protein backbone reveals mutants that react with the non-natural substrate O6-propargylguanine, libraries generated by conventional random or targeted saturation mutagenesis, by contrast, do not yield any mutants with activity towards this new substrate | Homo sapiens |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
O6-benzylguanine | - |
Homo sapiens |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Homo sapiens | - |
- |
- |
Purification (Comment) | Organism |
---|---|
mutants purified by either nickel or glutathione affinity chromatography | Homo sapiens |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | low reactivity of the non-natural substrate O6-propargylguanine with AGT, most likely arises from the poor binding of the substrate to the active site of AGT | Homo sapiens | ? | - |
? |
Synonyms | Comment | Organism |
---|---|---|
AGT | - |
Homo sapiens |
O6-alkylguanine-DNA alkyltransferase | - |
Homo sapiens |