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Literature summary for 2.1.1.6 extracted from

  • Tsuji, E.; Okazaki, K.; Isaji, M.; Takeda, K.
    Crystal structures of the apo and holo form of rat catechol-O-methyltransferase (2009), J. Struct. Biol., 165, 133-139.
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli BL21 Codon Plus (DE3) cells Rattus norvegicus

Crystallization (Commentary)

Crystallization (Comment) Organism
apo and holo forms of rat catechol-O-methyltransferase, hanging drop vapor diffusion method, the apo crystals are grown from 0.2 M (NH4)2SO4, 30% (w/v) PEG8000, the holo form of crystals are grown from 0.2 M (NH4)2SO4, 26% (w/v) PEG8000, 0.2% (v/v) sucrose Rattus norvegicus

Localization

Localization Comment Organism GeneOntology No. Textmining
soluble
-
Rattus norvegicus
-
-

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
24889
-
1 * 24889, ESI Q-pole mass spectrometry Rattus norvegicus
24890
-
calculated from amino acid sequence Rattus norvegicus
24890
-
ESI Q-pole mass spectrometry Rattus norvegicus
24891
-
1 * 24891, calculated from amino acid sequence Rattus norvegicus

Organism

Organism UniProt Comment Textmining
Rattus norvegicus P22734
-
-

Purification (Commentary)

Purification (Comment) Organism
GST Sepharose resin column chromatography, GSTrap column chromatography, Super Q-5PW column chromatography, and Superdex 75 gel filtration Rattus norvegicus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4-hydroxyestradiol + S-adenosyl-L-methionine
-
Rattus norvegicus S-adenosyl-L-homocysteine + ?
-
?

Subunits

Subunits Comment Organism
monomer 1 * 24889, ESI Q-pole mass spectrometry Rattus norvegicus
monomer 1 * 24891, calculated from amino acid sequence Rattus norvegicus

Synonyms

Synonyms Comment Organism
COMT
-
Rattus norvegicus
S-COMT soluble COMT Rattus norvegicus